Chlamydia trachomatis - Female (Direct Access Testing)


  • EPIC Code:
  • LAB3251
  • Soft Test Code:
Alternate Names
  • Chlamydia by Nucleic Acid Probe
  • Chlamydia Female Direct Access
  • Chlamydia trachomatis by Probe
  • Chlamydia, NAA; RNACT

Specimen Collection & Preparation

Specimen Requirements

Swab in in the Yellow PCR Media Tube

Alternate Specimen

Endocervical Swab in Pink Xpert Swab Specimen Collection Kit 

Female Cervical Collection:
  1.  Use CT/NG Vaginal/Endocervical Specimen Collection Kit (pink top transport tube)
  2. Remove excess mucus from the cervix using the large cleaning swab.  Discard this swab.
  3. Open the package containing the pink capped Swab Transport Tube and collection swab.
  4. Insert the collection swab into the endocervical canal and rotate 10-30 seconds.
  5. Withdraw the swab and place into the Transport tube.
  6. Break the swab shaft at the score line and securely recap the Transport tube.
  7. Label the transport tube with sample identification information including 2 unique patient identifiers.
  8. Send to the laboratory at room temperature.

8.0 mL Urine in a Yellow Capped Tube
  miniumum: 5.0 mL Urine
6.0 mL Urine in a Clear Red Top Tube - No Clot Activator
  miniumum: 5.0 mL Urine

Patient should not urinate for at least one hour prior to collecting the specimen.  Female patients should not cleanse the labial area prior to collecting the specimen.  Collect the first catch urine (approximately 20-50 mL of the initial urine stream) into a urine collection cup free of any preservatives.

Urine Collection:


Swab - 14 days Refrigerated (within 8 hours after collection)

Urine - 24 hours Ambient, 24 hours Refrigerated

Collection Instructions

Order must include the site and/or source of collection; Endocervical, Vaginal, Anorectal, or Oropharyngeal

Vagninal Collection

Anorectal (Rectal) Collection

Oropharyngeal (Throat) Collection

For Vaginal self collection patient instruction sheet see 
Addendum D
For Vaginal self collection Spanish patient instruction sheet see Addendum D
For Vaginal self collection Burmese patient instruction sheet see Addendum D
For Vaginal self collection Arabic patient instruction sheet see Addendum D

Clinical Interpretation

Reference Range:

Negative for Chlamydia trachomatis by amplified probe

Test Comments:

For patient less than 12 years of age - Culture is the only recommended procedure for diagnosing chlamydial or gonorrheal infection in cases of suspected child abuse.

  • Real-Time Polymerase Chain Reaction

Production Schedule

Sites Performed
  • Parkview Regional Medical Center
Days Performed
  • Molecular Diagnostics

Coding & Compliance



CPT Coding


Medical Necessity Documentation

Duplicate testing on this test is not acceptable per Medicare:  Testing to only be done every 14 days