Quest AD-Detect® Beta-Amyloid 42/40 Ratio, Plasma

Overview

  • EPIC Code:
  • MSOT
  • Soft Test Code:
  • 11786
  • Send Out Test Code:
  • LAB2639
Alternate Names
  • AB 42/40
  • AB40
  • AB42
  • ABeta 40
  • ABeta 42
  • ABeta 42/40
  • AD
  • Alzheimer's Disease (AD)
Included Tests

ABeta 40; ABeta 42; ABeta 42/40 Ratio


Clinical Significance

This LC-MS/MS assay measures beta-amyloid 40 (AB40) as well as AB42 in plasma, rather than AB42 alone. This permits the normalization of beta amyloid levels circulating in plasma in different patients. Using the ratio of beta-amyloid 42/beta-amyloid 40, improves sensitivity and specificity for detecting Alzheimer's disease.


Specimen Collection & Preparation

Specimen Overview

Quest Diagnostics does not perform this test for individuals under the age of 18.

Pre-test and post-test counseling is strongly recommended.

Specimen Requirements:

-OR-

1.2 mL Plasma from a Lavendar Top Tube - K2 EDTA in a Polycpropylene Tuve
Minimum Volume:
0.6 mL Plasma*

* This volume does not allow for repeat testing

Collection Instructions:

Collect blood into a K2 EDTA plasma tube and mix by inversion 10 times. 


Processing Instructions:

Centrifuge to separate plasma from cells within 1 hour of collection.

Carefully transfer plasma into a polypropylene tube or one of the Quest issued polypropylene pour-off/transfer tubes. Do not transfer to conical tube. 

 


Rejection Criteria:

Moderate or gross hemolysis

Specimen collected in a glass tube

Specimen collected in a polystyrene tube

Specimen collected in a polycarbonate tube


Transport and Storage:
  • Iced: If unable to centrifuge within 1 hour of collection, store tube on ice
  • Ambient (18-24°C): Unacceptable
  • Refrigerated (2-8°C): 72 Hours
  • Frozen (-20° C or colder): 32 Days

    Transport

    Store

  • Ultra Frozen (-70° C or colder or by dry ice): 77 Days

    Store

Clinical Interpretation

Reference Range:

ABeta 42 Not established pg/mL
ABeta 40 Not established pg/mL
ABeta 42/40 Ratio ≥0.170


Methodology:
  • Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS)
Clinical Significance

This LC-MS/MS assay measures beta-amyloid 40 (AB40) as well as AB42 in plasma, rather than AB42 alone. This permits the normalization of beta amyloid levels circulating in plasma in different patients. Using the ratio of beta-amyloid 42/beta-amyloid 40, improves sensitivity and specificity for detecting Alzheimer's disease.


Documentation

This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.


Production Schedule

Sites Performed
  • Quest - Chantilly to San Juan Capistrano
Days Performed
Monday
Tuesday
Wednesday
Thursday
Friday
Saturday
Departments
  • Sendouts - Miscellaneous
Turn Around Time

4 to 6 days


Coding & Compliance

CDM

00913333


CPT Coding

82233, 82234