Lymphocyte Subset Panel

Overview

  • EPIC Code:
  • LAB2458
  • Soft Test Code:
  • FLSUB
Alternate Names
  • Flow Cytometry Lymph Subset
  • Lymph Subset
  • Lymphocyte Immuonphenotyping
  • Lymphocyte Subset with CBC
  • Lymphocyte Subset, T & B Cell
  • Lymphocyte Subset, T Cell & B Cell
  • Natural Killer Cells
  • NK Cells
  • T & B Cell Subset
Included Tests

CD3; CD4; CD8; CD19; CD56; Helper/Suppressor Ratio; CBC [Hematocrit (Hct); Hemoglobin (Hgb); Mean Corpuscular Hemoglobin (MCH); Mean Corpuscular Hemoglobin Concentration (MCHC); Mean Corpuscular Volume (MCV); Platelet (PLT);  Red Blood Cell Count (RBC); Red Cell Distribution Width Standard Deviation (RDWSD);  Red Cell Distribution Width Coefficient Variation (RDWCV); White Blood Cell Count (WBC); WBC Differential]

 


Specimen Collection & Preparation

Specimen Requirements

4.0 mL Whole Blood in a Green Top Tube - Na Heparin 
and
3.0 mL Whole Blood in a Lavender Top Tube - EDTA


Transport And Storage

48 hours Ambient


Collection Instructions

 


Minimum Volume


Neonatal Volume

Clinical Interpretation

Reference Range:

Pediatric (less than 3 years)
CD3 (Mature T-cells): 55% - 82% / 2324 - 3295 cells/uL
CD4+/ CD3+(Helper T-cells): 38% - 46% / 1538 - 2213 cells/uL
CD8+ / CD3+ (Suppressor T-Cells): 8% - 31% / 351 - 2479 cells/uL
Helper/Suppressor Ratio: 1.2 - 6.2
CD 19 (B-cells): 11% - 45% / 430 - 3300 cells/uL
CD 56 (NK cells): 0% - 16% / 0 - 1200 cells/uL

Adult
CD3 (Mature T-cells): 61% - 91% / 558 - 2061cells/uL
CD4 (Helper T-cells): 32% - 65% / 487 - 1678 cells/uL
CD8 (Suppressor T-Cells): 13% - 39% / 201 - 1045 cells/uL
Helper/Suppressor Ratio:  0.9 - 3.7
CD 19 (B-cells): 4% - 25% / 30 - 550 cells/uL
CD 56 (NK cells): 1% - 29% / 0 - 800 cell/uL

See CBC for its Reference Ranges


Test Comments:

Complete Blood Count with differential (cpt 85025) maybe be reflexed and charged


Methodology:
  • Flow Cytometry (FC)

Production Schedule

Sites Performed
  • Parkview Regional Medical Center
Days Performed
Monday
Wednesday
Friday
Departments
  • Flow Cytometry
Turn Around Time

48 to 72 hours


Coding & Compliance

CDM

01396359; 01421022; 01425056; 01396360; 01430747


CPT Coding

85025 (Reflex 85007/85008) 86355, 86357, 86359, 86360