Bordetella Pertussis / Parapertussis DNA Qualitative

Overview

  • EPIC Code:
  • MISC
  • Soft Test Code:
  • MSOT
  • Send Out Test Code:
  • 11365
Alternate Names
  • B. pertussis/para DNA, QL Real-Time PCR
  • B.PERTUSSIS DNA, PCR
  • Bordetella pertussis/parapertussis DNA, Qual R-T P
Included Tests

Bordetella pertussis DNA; Bordetella parapertussis DNA


Clinical Significance

Bordetella pertussis is the cause of whooping cough that may occur in unimmunized individuals. B. parapertussis is a related organism that causes a similar, but milder, disease. Laboratory diagnosis may require both culture and serological confirmation, although culture is difficult.


Specimen Collection & Preparation

Specimen Requirements

Nasopheryngeal specimen in a Blue Capped mini-tipped E-Swab® (with Liquid Amies)


Transport And Storage

48 hours Ambient
7 days Refrigerated
30 days Frozen

Ship swab refrigerated (cold packs).


Collection Instructions

Swab tips may be polyester, rayon, nylon flocked. Flexible shafts must be used for proper collection. Accuracy of the test depends on the collection of ciliated epithelial cells material. Half the distance from the nostril to the base of the ear could be used as estimation of the site of collection. Gently insert swab into the nostril. Keep the swab near the septum floor of the nose while gently pushing into the posterior nasopharynx. Rotate swab 2-3 times and hold in place for few seconds for optimal collection. Collection of both nostrils is recommended but not required.

Nasalpharyngeal Collection Instructions


Minimum Volume

0.3 mL NP Aspirate/Wash


Neonatal Volume

Clinical Interpretation

Reference Range:

B. pertussis DNA: Not Detected
B. parapertussis DNA: Not Detected


Test Comments:

This test detects but does not distinguish between B. Pertussis and B. Holmesii.


Methodology:
  • Real-Time Polymerase Chain Reaction
Clinical Significance

Bordetella pertussis is the cause of whooping cough that may occur in unimmunized individuals. B. parapertussis is a related organism that causes a similar, but milder, disease. Laboratory diagnosis may require both culture and serological confirmation, although culture is difficult.


Production Schedule

Sites Performed
  • Quest - Chantilly
Days Performed
Sunday
Monday
Tuesday
Wednesday
Thursday
Friday
Saturday
Departments
  • Sendouts - Miscellaneous
Turn Around Time

1 to 2 days


Coding & Compliance

CDM

00913333


CPT Coding

87798 x 2