Bordetella pertussis DNA; Bordetella parapertussis DNA
Bordetella pertussis is the cause of whooping cough that may occur in unimmunized individuals. B. parapertussis is a related organism that causes a similar, but milder, disease. Laboratory diagnosis may require both culture and serological confirmation, although culture is difficult.
Nasopheryngeal specimen in a Blue Capped mini-tipped E-Swab® (with Liquid Amies)
0.3 mL of Nasopharyngeal aspirate collected in a sterile vial,
1 mL nasopharyngeal wash, aspirate , or turbinate, submitted in a sterile, plastic, leakproof vial (Nasopharyngeal aspirates: Instill 1-1.5 mL of nonbacteriostatic saline (pH 7.0) into one nostril. Flush a plastic catheter or tubing with 2-3 mL of saline. Insert the tubing into the nostril parallel to the palate. Aspirate nasopharyngeal secretions. Repeat this procedure for the other nostril. Combine aspirates into a sterile vial. Ship refrigerated (cold packs) or frozen.) Nasopharyngeal aspirate - Room temperature: 48 hours;Refrigerated: 8 days;Frozen: 30 days
Minimum: Swab in VCM Transport Medium (Swab in VCM Transport Medium (acceptable): Collect Respiratory specimens on rayon or Dacron® swab, or direct respiratory specimen such as BAL, respiratory aspirate, or sputum. Specimen source is required.)
Minimum: 2 mL of a respiratory sample, submitted in a sterile, plastic, leakproof container, frozen
Swab, submitted in UTM or M4 Miltiuse Viral Transport Medium, frozen
48 hours Ambient
7 days Refrigerated
30 days Frozen
Ship swab refrigerated (cold packs).
Swab tips may be polyester, rayon, nylon flocked. Flexible shafts must be used for proper collection. Accuracy of the test depends on the collection of ciliated epithelial cells material. Half the distance from the nostril to the base of the ear could be used as estimation of the site of collection. Gently insert swab into the nostril. Keep the swab near the septum floor of the nose while gently pushing into the posterior nasopharynx. Rotate swab 2-3 times and hold in place for few seconds for optimal collection. Collection of both nostrils is recommended but not required.
Nasalpharyngeal Collection Instructions
0.3 mL NP Aspirate/Wash
B. pertussis DNA: Not Detected
B. parapertussis DNA: Not Detected
This test detects but does not distinguish between B. Pertussis and B. Holmesii.
Bordetella pertussis is the cause of whooping cough that may occur in unimmunized individuals. B. parapertussis is a related organism that causes a similar, but milder, disease. Laboratory diagnosis may require both culture and serological confirmation, although culture is difficult.
1 to 2 days
00913333
87798 x 2