Herpes Simplex Virus 1 & 2

Overview

  • EPIC Code:
  • LAB2946
  • Soft Test Code:
  • HSDNA
Alternate Names
  • Herpes Simplex 1+2 by DNA Amplification
  • Herpes Simplex, HSV 1+2
  • HSV-1 & HSV-2
Included Tests

Herpes Simplex Virus 1 (HSV-1); Herpes Simplex Virus 2 (HSV-2);


Specimen Collection & Preparation

Specimen Requirements

Lesion in M4RT Vial 
   Cutaneous - skin, penis, labia
   or
   Mucrocutaneous - oral, ocular, urethral, nasal, vaginal, cervical, rectal



Transport And Storage

48 hours Ambient (up to 30 degrees C)
7 days Refrigerated (Transport)
7 days Frozen


Collection Instructions

Specimen must be collected from Cutaneous (skin, penis, labia) or Mucrocutaneous (oral, ocular, urethral, nasal, vaginal, cervical, rectal) lesions from symptomatic patients and immediately placed in Remel Viral Transport Media - Room Temp (M4RT).

Collection site and/or source must be given on the order. 


Endocervical:
1. Remove excess mucous from cervial os and surrounding mucosa with large tipped swab.
2. Insert specimen collection swab into endocervical canal.
3. Gently rotate swab for 10-30 seconds to ensure adequate sampling.
4. Withdraw swab carefully to avoid vaginal mucosa.
5. Remove cap from transport vial and place swab into vial.
6. Snap off swab shaft, re-cap transport vial.

Urethral:
1. The patient should not have urinated for at least 1 hour prior to collection.
2. Insert collection swab 2-4 cm into urethra.
3. Gently rotate swab for 2-3 seconds to ensure adequate sampling.
4. Withdraw swab carefully.
5. Remove cap from transport vial and place swab into vial.
6. Snap off swab shaft, re-cap transport vial.


Minimum Volume


Neonatal Volume

Clinical Interpretation

Reference Range:

 No HSV-1 or HSV-2 DNA detected.


Methodology:
  • Helicase-Dependant DNA Amplification

Production Schedule

Sites Performed
  • Parkview Regional Medical Center
Days Performed
Monday
Tuesday
Wednesday
Thursday
Friday
Departments
  • Microbiology
Turn Around Time

1 to 3 days


Coding & Compliance

CDM

01428529


CPT Coding

87529