Overview

  • EPIC Code:
  • LAB342
  • Soft Test Code:
  • FTCEL
Alternate Names
  • CD4
  • Flow Cytometry T Cell Subset
  • Lymph Subset (CD4/TCELL)
  • T Cell Subset with CBC
  • T-Cell Subset with CBC
  • TCell Subset with CBC
Included Tests

CD3; CD4; CD8; Helper/Suppressor Ratio; CBC [Automated Differential; Hematocrit (Hct); Hemoglobin (Hgb); Mean Corpuscular Hemoglobin (MCH); Mean Corpuscular Hemoglobin Concentration (MCHC); Mean Corpuscular Volume (MCV); Platelet (PLT);  Red Blood Cell Count (RBC); Red Cell Distribution Width Standard Deviation (RDWSD);  Red Cell Distribution Width Coefficient Variation (RDWCV); White Blood Cell Count (WBC]


Clinical Significance

To achieve subsequent CD4+ T-Cell counts after baseline values have previously been established.


Specimen Collection & Preparation

Client Notes:

 CBCWD must be run within 24 hours of FTCEL testing


Specimen Requirements

3.0 mL Whole Blood in a Green Top Tube - Na Heparin 
and
3.0 mL Whole Blood in a Lavender Top Tube - EDTA

Alternate Specimen

Alternate for the Na Heparin
  3.0 mL Whole Blood in a Lavender Top Tube - EDTA,

TransportAndStorage

48 hours Ambient

Collection Instructions


Clinical Interpretation

Reference Range:

Pediatric (less than 3 years)
CD3 (Mature T-cells): 55% - 82% / 2324 - 3295 cells/uL
CD4 (Helper T-cells): 38% - 46% / 1538 - 2213 cells/uL
CD8 (Suppressor T-Cells): 8% - 31% / 351 - 2479 cells/uL
CD4/CD8 Ratio: 1.2 - 6.2

Adult
CD3 (Mature T-cells): 61% - 91% / 558 - 2061 cells/uL
CD4 (Helper T-cells): 32% - 65% / 487 - 1678 cells/uL
CD8 (Suppressor T-Cells): 13% - 39% / 201 - 1045 cells/uL
CD4/CD8 Ratio: 0.9 - 3.7

See CBC for its Reference Ranges


Test Comments:

A complete Blood count will be reflexed and charged 


Methodology:
  • Flow Cytometry (FC)

Clinical Significance

To achieve subsequent CD4+ T-Cell counts after baseline values have previously been established.


Documentation

The CDC Guidelines for the performance of CD4+ T-Cell Determinations in persons with Human Immunodeficiency Virus Infection recommends the Lymphocyte Subset Panel to achieve baseline quantitations for T&B cell populations. MMWR Vol 41/No. RR-8, 1992.


Production Schedule

Sites Performed
  • Parkview Regional Medical Center
Days Performed
Monday
Wednesday
Friday
Saturday
Departments
  • Flow Cytometry
Turn Around Time

48 to 72 hours


Coding & Compliance

CDM

01396359; 01396360; 01430747


CPT Coding

86359, 86360, 85025 (Reflex 85007, 85008)