Clostridium difficile Pathway

Overview

  • EPIC Code:
  • LAB3208
  • Soft Test Code:
  • CDMT
Alternate Names
  • C diff
  • C Diff Pathway
  • C-Diff Pathway
  • C. diff Toxin
  • C. DIFFICILE MOLECULAR W/ TOXIN EIA REFLEX
  • cdiff
  • CDiff Pathway
  • Clostridum difficile
  • GDH
  • Toxins
Clinical Significance

Clostridium difficile infection is a serious disease causing diarrhea, colitis, and even pseudomembranous colitis that develops as a result of antibiotic treatment. It may also occur due to nosocomial transmission. Recently, community acquired infections have been found with patients who have not received antibiotics. It is important that true cases be identified quickly to allow treatment which will prevent serious sequelae for the patient and possible nosocomial transmission.


Specimen Collection & Preparation

Patient Preparation

Patients must be symptomatic. 
 No "test of cure" specimens should be submitted.  Patients may remain positive for long periods without symptoms.


Specimen Requirements

5.0 mL unformed Feces in a Non-Sterile Yellow Capped Container


Click Below for Temporatry specimen needs and information 
 This the link to the Quest test directory for Cdiff toxin/GDH with reflex to PCR test:

 https://testdirectory.questdiagnostics.com/test/test-detail/91664/clostridium-difficile-toxingdh-with-reflex-to-pcr?p=r&q=91664&cc=AMD


Alternate Specimen

 

TransportAndStorage

≤72 hours refrigerated
>72 hours frozen.

Collection Instructions

Only specimens that conform to the container shape will be tested.

Clinical Interpretation

Reference Range:

Negative for toxigenic Clostridium difficile by DNA amplification. Positive for toxigenic Clostridium difficile by DNA amplification.
Negative for Toxins A & B by EIA. Positive for Toxins A & B by EIA.


Test Comments:

Molecular testing (for the tcdB Toxin B gene of toxigenic Clostridium difficile) is performed initially to determine presence of organism genome. Only a positive molecular test will reflex EIA testing for Toxins A & B


Methodology:
  • Molecular PCR, Toxin EIA

Clinical Significance

Clostridium difficile infection is a serious disease causing diarrhea, colitis, and even pseudomembranous colitis that develops as a result of antibiotic treatment. It may also occur due to nosocomial transmission. Recently, community acquired infections have been found with patients who have not received antibiotics. It is important that true cases be identified quickly to allow treatment which will prevent serious sequelae for the patient and possible nosocomial transmission.


Production Schedule

Sites Performed
  • Parkview Regional Medical Center
Days Performed
Sunday
Monday
Tuesday
Wednesday
Thursday
Friday
Saturday
Departments
  • Microbiology
Turn Around Time

8 to 12 hours


Coding & Compliance

CDM

01425522


CPT Coding

87324


Medical Necessity Documentation

Testing may only be performed after 7 for original negative, and  after 10 days for original positive