Overview
EPIC Code:
- LAB2785
Soft Test Code:
- CJD
Send Out Test Code:
- 37989
Alternate Names
- 14-3-3 (ELISA), Total TAU (ELISA) and RT-QuIC Panel
- 14-3-3 Protein, CSF
- 14-3-3 Protein, Spinal Fluid
- CJD
- Cretuzfeld-Jakob
- Creutzfeldt-Jakob 14-3-3 Protein
- Protein 14-3-3
Clinical Significance
May aid in diagnosis of prion disease.
Assumes that the patient has a history of a rapid neurodegenerative condition and does not have several conditionsi that might compromise the test results.
Specimen Collection & Preparation
Test Notes:
Patient must be 12 years of age or older.
Required Forms & Information:
Complete National Prion Disease Requisition
Clinical History required to be filled out by ordering provider
Specimen Overview
If suspected CJD, CSF samples MUST be labeled as " Suspected CJD"
For more information please refer to the Creutzfeldt-Jakob Disease policy.
Due to stability time constraints avoid collections on Friday & Saturday
Specimen Requirements:
-OR-
2.0 mL Cerbrospinal Fluid (CSF) in Sterile Container
Collection Instructions:
Collect CSF by lumbar puncture
Discard first 2.0 mL that flows from tap
Collect next 2.0 mL to 5.0 mL CSF, avoiding bloody tap.
Processing Instructions:
Freeze within 20 minutes of collection
Rejection Criteria:
Bloody and insufficient sample quantity
Samples that are colored or contain blood cannot be performed for RT-QuIC and 14-3-3 GAMMA
Transport and Storage:
-
Ambient:
Unacceptable
-
Refrigerated:
Unacceptable
-
Frozen (-20 C or colder):
Indefinetly
The sample must be immediately frozen, at least in a -20° C freezer
*** Ship Monday through Wednesday (priority overnight) ***
Ship on dry ice using a styrofoam container with sufficient dry ice (5 lbs/24 hours).
Clinical Interpretation
Reference Range:
Refer to Interpretive Results
Methodology:
- Immunoassay (IA)
- Real-Time Quaking-Induced Conversion (RT-QuIC)
Clinical Significance
May aid in diagnosis of prion disease.
Assumes that the patient has a history of a rapid neurodegenerative condition and does not have several conditionsi that might compromise the test results.
Production Schedule
Sites Performed
- Quest - Chantilly to National Prion Lab
Days Performed
Tuesday
Thursday
Departments
- Sendouts - Clinical
- Blood Bank
Turn Around Time
7 days
Coding & Compliance
CDM
00913960 (x2); 00913959
CPT Coding
86317 (14-3-3 & TAU), 0035U (RT-QuIC)