14-3-3 Protein, CSF (Creutzfeldt-Jakob)

Overview

  • EPIC Code:
  • LAB2639
  • Soft Test Code:
  • Send Out Test Code:
  • 37989
Alternate Names
  • 14-3-3 (ELISA), Total TAU (ELISA) and RT-QuIC Panel
  • 14-3-3 Protein, CSF
  • 14-3-3 Protein, Spinal Fluid
  • CJD
  • Cretuzfeld-Jakob
  • Creutzfeldt-Jakob 14-3-3 Protein
  • Protein 14-3-3
Included Tests

T-tau protein (CSF)

14-3-3 GAMMA (CSF)

RT-QulC (CSF)


Clinical Significance

The 14-3-3 protein is a marker for some prion diseases, such as Creutzfeldt-Jakob disease (CJD), when a number of other neurodegenerative conditions are excluded.
Assumes that the patient has a history of a rapid neurodegenerative condition and does not have several conditions that might compromise the test results.


Specimen Collection & Preparation

Test Notes

Patient must be 12 years of age or older.


Required Forms & Information

Complete National Prion Disease Requisition

Clinical History required to be filled out by ordering provider 


Specimen Requirements:

-OR-

2.0 mL Cerebrospinal Fluid (CSF) collected in a sterile screw-cap vial
Minimum Volume:
1 mL CSF*

* This volume does not allow for repeat testing

Collection Instructions:

Collect CSF by lumbar puncture
Discard first 2.0 mL that flows from tap
Collect next 2.0 mL to 5.0 mL CSF, avoiding bloody tap.

If suspected CJD, CSF samples MUST be labeled as " Suspected CJD"  


Rejection Criteria:

Bloody and insufficient sample quantity

Samples that are colored or contain blood cannot be performed for RT-QuIC and 14-3-3 GAMMA


Transport and Storage:
  • Ambient (18-24°C): Unacceptable
  • Refrigerated (2-8°C): Unacceptable
  • Frozen (-20° C or colder): Indefinitely

    The sample must be immediately frozen, in a -20° C freezer

    Ship on dry ice using a styrofoam container with sufficient dry ice (5 lbs/24 hours).

SHIP MONDAY THROUGH WEDNESDAY (PRIORITY OVERNIGHT)

Clinical Interpretation

Reference Range:

Refer to Interpretive Results 


Methodology:
  • Immunoassay (IA)
  • Real-Time Quaking-Induced Conversion (RT-QuIC)
Clinical Significance

The 14-3-3 protein is a marker for some prion diseases, such as Creutzfeldt-Jakob disease (CJD), when a number of other neurodegenerative conditions are excluded.
Assumes that the patient has a history of a rapid neurodegenerative condition and does not have several conditions that might compromise the test results.


Production Schedule

Sites Performed
  • Quest - Chantilly to National Prion Lab
Schedule Frequency
Setup schedule varies
Departments
  • Sendouts - Clinical
Turn Around Time

7 days


Coding & Compliance

CDM

00913960 (x2); 00913959


CPT Coding

0035U, 83520, 84394