14-3-3 Protein, CSF (Creutzfeldt-Jakob)

Overview

  • EPIC Code:
  • LAB2785
  • Soft Test Code:
  • CJD
  • Send Out Test Code:
  • 37989
Alternate Names
  • 14-3-3 (ELISA), Total TAU (ELISA) and RT-QuIC Panel
  • 14-3-3 Protein, CSF
  • 14-3-3 Protein, Spinal Fluid
  • CJD
  • Cretuzfeld-Jakob
  • Creutzfeldt-Jakob 14-3-3 Protein
  • Protein 14-3-3
Clinical Significance

May aid in diagnosis of prion disease.
Assumes that the patient has a history of a rapid neurodegenerative condition and does not have several conditionsi that might compromise the test results.


Specimen Collection & Preparation

Required Forms & Information:

Complete National Prion Disease Requisition

Clinical History required to be filled out by ordering provider 

Patient Preparation

Patient must be 12 years of age or older.


Specimen Overview

If suspected CJD, CSF samples MUST be labeled as " Suspected CJD"  

For more information please refer to the Creutzfeldt-Jakob Disease policy. 

Due to stability time constraints avoid collections on Friday & Saturday

Specimen Requirements:

-OR-

2.0 mL Cerbrospinal Fluid (CSF) in Sterile Container
Collection Instructions:

Collect CSF by lumbar puncture
Discard first 2.0 mL that flows from tap
Collect next 2.0 mL to 5.0 mL CSF, avoiding bloody tap.
Freeze within 20 minutes of collection


Rejection Criteria:

Bloody and insufficient sample quantity.


Transport and Storage:
  • Ambient: 24 Hours
  • Refrigerated: 14 Days
  • Frozen (-20 C or colder): Indefinetly

    Transport 

    Ship on dry ice

Clinical Interpretation

Reference Range:

For Non-Prion Disease

RT-QuIC: Negative
Total TAU (ELISA): 0-1149 pg/mL
14-3-3 GAMMA (ELISA): <173 - 1999 AU/mL.

Likelihood of Prion Disease

RT-QuIC Positive: >98%
RT-QuIC Negative & Total TAU <499 = <0.2%
RT-QuIC Negative & Total TAU 500-2499 = <1%


Methodology:
  • Quantitative Enzyme-Linked Immunosorbent Assay (ELISA)
  • Real-Time Quaking-Induced Conversion (RT-QuIC)

Clinical Significance

May aid in diagnosis of prion disease.
Assumes that the patient has a history of a rapid neurodegenerative condition and does not have several conditionsi that might compromise the test results.


Production Schedule

Sites Performed
  • Quest - Chantilly to National Prion Lab
Days Performed
Tuesday
Thursday
Departments
  • Sendouts - Clinical
  • Blood Bank
Turn Around Time

21 days


Coding & Compliance

CDM

00913960 (x2); 00913959


CPT Coding

86317 (14-3-3 & TAU), 0035U (RT-QuIC)