25-Hydroxyvitamin D (D2, D3)

Overview

  • EPIC Code:
  • LAB2524
  • Soft Test Code:
  • MSOT
  • Send Out Test Code:
  • 92888
Alternate Names
  • Quest Assure D
  • Quest Assure D 25-Hydroxyvitamin D (D2,D3), LC/MS/
  • Quest Assure D™ 25-Hydroxyvitamin D (D2, D3), LC/M
  • QuestAssure D 25-Hydroxyvitamin D (D2,D3), LC/MS/M
  • QuestAssureD™ 25-Hydroxyvitamin D (D2, D3), LC/MS/
  • Vit D LC/MS/MS
  • Vitamin D LC/MS/MS
  • Vitamin D, 25 OH Total, D2, D3
  • Vitamin D, 25-OH, LC/MS/MS
  • Vitamin D2, 25-hydroxy
  • Vitamin D3, 25-hydroxy
Included Tests

Vitamin D, 25-OH, Total; Vitamin D, 25-OH, D3; Vitamin D, 25-OH, D2


Clinical Significance

Measurement of serum 25-OH vitamin D concentrations provide a good index of circulating vitamin D activity in patients not suffering from renal disease. Lower than normal 25-OH vitamin D levels can result from a dietary deficiency, poor absorption of the vitamin or impaired metabolism of the sterol in the liver. A 25-OH vitamin D deficiency can lead to bone diseases such as rickets and osteomalacia. Above normal levels can lead to hypercalcemia. This assay employs liquid chromatography tandem mass spectrometry to independently measure and report the two common forms of 25-hydroxy vitamin D: 25-OH D3 - the endogenous form of the vitamin and 25-OH D2 - the analog form used to treat 25-OH Vitamin D3 deficiency.


Specimen Collection & Preparation

Patient Preparation

Fasting preferred, but not required


Specimen Requirements

1.0 mL Serum from a Red Top Tube in a Plastic Vial

Alternate Specimen

1.0 mL Serum from a SST Gold Top Tube

TransportAndStorage

21 days Ambient - Transport
21 days Refrigerated
21 days Frozen

Collection Instructions

Allow blood to clot at room temperature. Centrifuge and separate the serum from the cells immediately.

Clinical Interpretation

Reference Range:

Vitamin D, 25-OH, Total 30-100 ng/mL
Vitamin D, 25-OH, D3 Not established
Vitamin D, 25-OH, D2 Not established


Methodology:
  • Liquid Chromatography / Tandem Mass Spectrometry (LC-MS/MS)

Clinical Significance

Measurement of serum 25-OH vitamin D concentrations provide a good index of circulating vitamin D activity in patients not suffering from renal disease. Lower than normal 25-OH vitamin D levels can result from a dietary deficiency, poor absorption of the vitamin or impaired metabolism of the sterol in the liver. A 25-OH vitamin D deficiency can lead to bone diseases such as rickets and osteomalacia. Above normal levels can lead to hypercalcemia. This assay employs liquid chromatography tandem mass spectrometry to independently measure and report the two common forms of 25-hydroxy vitamin D: 25-OH D3 - the endogenous form of the vitamin and 25-OH D2 - the analog form used to treat 25-OH Vitamin D3 deficiency.


Production Schedule

Sites Performed
  • Quest - Chantilly
Days Performed
Monday
Tuesday
Wednesday
Thursday
Friday
Saturday
Departments
  • Sendouts - Miscellaneous
Turn Around Time

2 to 3 days


Coding & Compliance

CDM

00913333


CPT Coding

82306