Asparaginase Activity; Asparaginase Antibody
Used to monitor Asparaginase therapy for sufficient enzyme activity, to assess silent inactivation by patient sensitization (activity assay). To assess if a patient is producing antibodies against asparaginase (antibody assay).
Asparaginase is an enzyme produced by some plants, animals, and bacteria that hydrolyzes the amino acid asparagine to aspartic acid. It is commonly produced in native forms from Escherichia coli or Erwinia chrysanthemi and has a molecular weight of 138-141 kDa. PEG-modified E.coli has a MW of 145 kDa.
Minimum Required Order & Billing information
All specimens and requisitions must be labeled at the time of collection with at least two patient identifiers, which must also be indicated on the requisition.
• Tubes must be labeled with at least a minimum of two patient identifiers. A single tube of serum (containing a minimum of 0.5 mL of sample volume) may be submitted for use for both assays.
• Two patient identifiers (e.g., full name and DOB, medical record number, or social security number)
• Patient gender
• Patient date of birth
• Name and address of physician or facility ordering the test
• Ordering Physician name
• Date/Time of specimen collection
• Type of L-asparaginase drug administered, if known
• Test(s) requested (Asparaginase Activity, or Asparaginase Panel)
• Provide complete instructions for direct bill including address and contact information
2.0 mL Serum from a Red Top Tube in a Plastic Vial
2.0 (0.5) mL Serum from a SST Gold Top Tube
1 day Ambient
3 days Refrigerated
30 days Frozen
Only 3 Freeze/Thaw cycles
Freeze prior to transport
Separate serum from RBCs within 2 hours, freeze/refrigerate serum immediately
No samples will be processed after 3 pm Monday through Friday
0.5 mL Serum
Physician established therapeutic range.
Used to monitor Asparaginase therapy for sufficient enzyme activity, to assess silent inactivation by patient sensitization (activity assay). To assess if a patient is producing antibodies against asparaginase (antibody assay).
Asparaginase is an enzyme produced by some plants, animals, and bacteria that hydrolyzes the amino acid asparagine to aspartic acid. It is commonly produced in native forms from Escherichia coli or Erwinia chrysanthemi and has a molecular weight of 138-141 kDa. PEG-modified E.coli has a MW of 145 kDa.
Results are reported in IU/mL (activity assay). Traditional antigen-capture ELISA assay. Results are reported as positive or negative for antibodies (antibody assay).
1 day
82657 for the activity assay (If drug administered is not specified then results will be reported for Oncaspar, Erwinaze and Rylaze which warrants 3 units, 82657*3). Assay is 83516