Asparaginase Panel

Overview

  • EPIC Code:
  • Soft Test Code:
  • MSOT
Alternate Names
  • L-Asparaginase
Included Tests

 Asparaginase Activity; Asparaginase Antibody


Clinical Significance

Used to monitor Asparaginase therapy for sufficient enzyme activity, to assess silent inactivation by patient sensitization (activity assay). To assess if a patient is producing antibodies against asparaginase (antibody assay).

Asparaginase is an enzyme produced by some plants, animals, and bacteria that hydrolyzes the amino acid asparagine to aspartic acid. It is commonly produced in native forms from Escherichia coli or Erwinia chrysanthemi and has a molecular weight of 138-141 kDa. PEG-modified E.coli has a MW of 145 kDa. 


Specimen Collection & Preparation

Client Notes:

  Minimum Required Order & Billing information 
All specimens and requisitions must be labeled at the time of collection with at least two patient identifiers, which must also be indicated on the requisition.
• Tubes must be labeled with at least a minimum of two patient identifiers. A single tube of serum (containing a minimum of 0.5 mL of sample volume) may be submitted for use for both assays.
• Two patient identifiers (e.g., full name and DOB, medical record number, or social security number)
• Patient gender
• Patient date of birth
• Name and address of physician or facility ordering the test
• Ordering Physician name
• Date/Time of specimen collection
• Type of L-asparaginase drug administered, if known
• Test(s) requested (Asparaginase Activity, or Asparaginase Panel)
• Provide complete instructions for direct bill including address and contact information


Specimen Requirements

2.0 mL Serum from a Red Top Tube in a Plastic Vial

Alternate Specimen

2.0 (0.5) mL Serum from a SST Gold Top Tube

TransportAndStorage

1 day Ambient
3 days Refrigerated
30 days Frozen

Only 3 Freeze/Thaw cycles

Freeze prior to transport 

Collection Instructions

Separate serum from RBCs within 2 hours, freeze/refrigerate serum immediately

No samples will be processed after 3 pm Monday through Friday

Clinical Interpretation

Reference Range:

 Physician established therapeutic range.


Methodology:
  • Spectrophotometry / Absorbance-based enzyme-coupled kinetic reaction

Clinical Significance

Used to monitor Asparaginase therapy for sufficient enzyme activity, to assess silent inactivation by patient sensitization (activity assay). To assess if a patient is producing antibodies against asparaginase (antibody assay).

Asparaginase is an enzyme produced by some plants, animals, and bacteria that hydrolyzes the amino acid asparagine to aspartic acid. It is commonly produced in native forms from Escherichia coli or Erwinia chrysanthemi and has a molecular weight of 138-141 kDa. PEG-modified E.coli has a MW of 145 kDa. 


Documentation

Results are reported in IU/mL (activity assay). Traditional antigen-capture ELISA assay. Results are reported as positive or negative for antibodies (antibody assay).


Production Schedule

Sites Performed
  • Granger Genetics
Turn Around Time

1 day


Coding & Compliance

CPT Coding

82657 for the activity assay (If drug administered is not specified then results will be reported for Oncaspar, Erwinaze and Rylaze which warrants 3 units, 82657*3). Assay is 83516