Adrenocorticotropic Hormone Stimulation Test


  • EPIC Code:
  • LAB711
  • Soft Test Code:
Alternate Names
  • ACTH Short Stimulation Test
  • ACTH Stimulation (4 samples)
  • ACTH Stimulation (Pediatric Oncology)
  • ACTH Stimulation Test
  • Addison Disease Testing
  • Adrenocorticotropic Hormone Stimulation Test (4 Samples)
  • Cortrosyn Stimulation
  • Cosyntropin
  • Cosyntropin Stimulation
Included Tests

Baseline Cortisol; 20 minute Cortisol; 40 minute Cortisol; 60 minute Cortisol

Clinical Significance

Method for testing the adrenal reserve to rule out Addison Disease

Specimen Collection & Preparation

Client Notes:

Synthetic ACTH infections are performed by the PPG Pediatric Hematology & Oncology Clinic

Outside of PPG Pediatric Hematology & Oncology Clinic, synthetic ACTH Injections are performed at Parkview Randallia Hospital Infusion Center Only PVH Intake Form

Patient Preparation

Glucocorticoids should not be taken prior to an ACTH Stimulation Test.  The ordering provider will provide instructions to the patient detailing the length of time glucocorticoids should be withheld.  Questions about compliance with these instructions should be referred to the ordering provider.

Specimen Requirements:


Four - 1.0 mL Serum in a SST Gold Top Tube(s) - One for each timed draw
Minimum Volume:
0.5 mL Serum for each specimen
Collection Instructions:

  1. A base line level Cortisol to be drawn.
  2. Within 30 minutes of baseline being drawn, the patient is to be injected with a dose of synthetic ACTH, Cortrosyn, IM. (Appropriate dosage is determined by the ordering provider)
  3. Start time the test after the injection has been given.
  4. Collect a timed specimen at 20 minutes after injection.
  5. Collect a timed specimen at 40 minutes after injection.
  6. Collect a timed specimen at 60 minutes after injection.

Collection interval (Baseline, 20 min, 40 min, 60 min) must be identified on the specimen label, along with the patient’s full legal name, a unique identifier (the unique identifier may be the DOB or the requisition number), date and time of collection, and the phlebotomist’s initials.

Transport and Storage:
  • Refrigerated: 48 Hours

Clinical Interpretation

Reference Range:

No reference range established

  • Chemiluminescence (CL)

Clinical Significance

Method for testing the adrenal reserve to rule out Addison Disease

Production Schedule

Sites Performed
  • Parkview Regional Medical Center
Days Performed
  • Chemistry

Coding & Compliance


01483971 x 4

CPT Coding

82533 x 4