Cytomegalovirus DNA, Quantitative

Overview

  • EPIC Code:
  • LAB913
  • Soft Test Code:
  • CYQNT
  • Send Out Test Code:
  • 12190
Alternate Names
  • CMV DNA
  • CMV DNA Ultraquant
  • CMV DNA, Quantitative
  • Cyto DNA Ultra Quant
  • Cytomegalovirus (CMV) DNA, Quant RT PCR
  • Cytomegalovirus DNA UltraQuant
  • Cytomegalovirus DNA, Quantitative Real-Time PCR
Clinical Significance

In patients who are immunocompromised, CMV may cause disseminated, severe disease. CMV is also the most common cause of congenital viral infection in humans. Quantitative PCR methods may be useful in monitoring CMV replication in immunosuppressed patients or in determining the viral load of CMV in amniotic fluid. This is a quantitative molecular test, with a linear range of 200-2,000,000 copies/mL.


Specimen Collection & Preparation

Specimen Overview

For any specimen collected must specify source on the test requisition form and specimen container.

Specimen Requirements:

-OR-

1.0 mL Whole Blood in Lavender Top Tubes - EDTA
Minimum Volume:
0.5 ML Whole Blood*

* This volume does not allow for repeat testing

Collection Instructions:

Collect whole blood in sterile tubes containing EDTA as anticoagulant.


Rejection Criteria:

Blood collected in tubes with heparin anticoagulant is not accepted for this test. 

Frozen Specimen


Transport and Storage:
  • Ambient: 48 Hours
  • Refrigerated: 8 Days
  • Frozen (-20 C or colder): Unacceptable

-OR-

1.0 mL CSF from a Lavender Top Tube - EDTA in a Sterile Vial
Minimum Volume:
0.3 mL CSF*

* This volume does not allow for repeat testing

Collection Instructions:

Collect in a sterile container


Transport and Storage:
  • Refrigerated:

    Store

  • Frozen (-20 C or colder):

    Store

-OR-

1.0 (0.3) mL Amniotic Fluid from a Lavender Top Tube - EDTA in a Plastic Vial
Minimum Volume:
0.3 mL Amniotic Fluid*

* This volume does not allow for repeat testing

Collection Instructions:

Collect in a sterile container


Transport and Storage:
  • :
  • Refrigerated:

    Store

  • Frozen (-20 C or colder):

    Store

-OR-

1.0 (0.3) mL Amniotic Fluid Supernatant
Minimum Volume:
0.3 mL Amniotic Supernate*

* This volume does not allow for repeat testing

Specimen Information:

Collect in a sterile container


Transport and Storage:
  • Refrigerated:

    Store

  • Frozen (-20 C or colder):

    Store

-OR-

1.0 mL Urine in a Yellow Capped Conical Tube or Plastic Container
Minimum Volume:
0.3 mL Urine*

* This volume does not allow for repeat testing

Specimen Information:

No preservative


Collection Instructions:

Collect in a sterile container 


Transport and Storage:
  • Refrigerated:

    Store

  • Frozen (-20 C or colder):

    Store

-OR-

1.0 mL Bronchoalveolar Lavage
Minimum Volume:
0.3 mL Bronchoalveolar Lavage*

* This volume does not allow for repeat testing

-OR-

1.0 mL Eye Fluid
Minimum Volume:
0.3 mL Eye Fluid*

* This volume does not allow for repeat testing

Clinical Interpretation

Reference Range:

< 200 copies/mL


Methodology:
  • Real-Time Polymerase Chain Reaction

Clinical Significance

In patients who are immunocompromised, CMV may cause disseminated, severe disease. CMV is also the most common cause of congenital viral infection in humans. Quantitative PCR methods may be useful in monitoring CMV replication in immunosuppressed patients or in determining the viral load of CMV in amniotic fluid. This is a quantitative molecular test, with a linear range of 200-2,000,000 copies/mL.


Production Schedule

Sites Performed
  • Quest - Chantilly
Departments
  • Sendouts - Clinical
Turn Around Time

3 to 6 days


Coding & Compliance

CDM

00917497


CPT Coding

87497