Acute Flaccid Myelitis (AFM)


  • EPIC Code:
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Specimen Collection & Preparation

Specimen Requirements

2  (> 1) Grams Whole Stool in a Sterile Gray 

Alternate Specimen

Specimen Type Minimum Amount Collection Storage Shipping Comments
Cerebrospinal fluid (CSF) 1 mL Spun and processed; standard cryovial tube; collect at same time or within 24 hours of serum if feasible Freeze at -20°C Ship on dry ice CSF will be used for special studies; EV/RV testing will be batched and results returned as sample amount allows
Serum* 0.4 mL Spun and processed; Tiger/red top tube; collect at same time or within 24 hours of CSF if feasible. Freeze at -20°C Ship on dry ice Serum will be used for special studies; no individual results will be returned
Respiratory – nasopharyngeal (NP) or oropharyngeal (OP) swab 1 ml Store in viral transport medium Freeze at -20°C Ship on dry ice EV/RV testing and typing will be performed and results returned within 10 days of sample receipt
In the event of death, please send the following specimens, if possible
Fresh-frozen tissue   Place directly on dry ice or liquid nitrogen Freeze at -70°C Ship on dry ice Representative sections from various organs are requested, but particularly from brain/spinal cord (including gray and white matter), heart, lung, liver, kidney, and other organs as available.
Formalin-fixed or formalin-fixed, paraffin-embedded tissue   Avoid prolonged fixation—tissues should have been fixed in formalin for 3 days, then transferred to 100% ethanol Room temperature Ship at room temperature with paraffin blocks in carriers to prevent breakage See comment above regarding frozen tissue


Freeze at -20°C
Ship on dry ice

Collection Instructions

Collect in sterile container, no special medium required. Please do not send a rectal swab

Two samples total, collected at least 24 hours apart, both collected as early in illness as possible and ideally within 14 days of illness onset. Results for EV/RV and poliovirus testing will be returned as testing completed (within 14 days)

Clinical Interpretation

Reference Range:

See Interpretive Report

  • Unknown

Production Schedule

Coding & Compliance

CPT Coding