Acute Leukemia Panel

Overview

  • EPIC Code:
  • LAB2452
  • Soft Test Code:
  • FACUT
Alternate Names
  • Acute Leukemia Panel, Flow Cytometry
  • Flow Cytometry, Acute
Included Tests

CBC [Automated Differential; Hematocrit (Hct); Hemoglobin (Hgb); Mean Corpuscular Hemoglobin (MCH); Mean Corpuscular Hemoglobin Concentration (MCHC); Mean Corpuscular Volume (MCV); Platelet (PLT);  Red Blood Cell Count (RBC); Red Cell Distribution Width Standard Deviation (RDWSD);  Red Cell Distribution Width Coefficient Variation (RDWCV); White Blood Cell Count (WBC)]; CD2; CD3; CD4; CD5; CD7; CD8; CD10; CD11b; CD13; CD14; CD15; CD16; CD19; CD20; CD22; CD23; CD33; CD34; CD36; CD38; CD41; CD45; CD56; CD57; CD64; CD71; CD79b; CD117; HLA-DR; Surface Kappa; Surface Lambda

 


Clinical Significance

Useful for distinguishing acute lymphoblastic leukemia from acute myelogenous leukemia and phenotypic subtyping of acute leukemias


Specimen Collection & Preparation

Specimen Requirements

4.0 mL Whole Blood in a Green Top Tube - Na Heparin
and 
3.0 mL Whole Blood in a Lavender Top Tube - EDTA


Alternate Specimen

Alternate for the Na Heparin
  3.0 mL Whole Blood in a Lavender Top Tube - EDTA,

When a Bone Marrow is submitted a copy of the requisition must be submitted.
1.0 mL Bone Marrow Aspirate in a Green Top Tube - Na Heparin 
or 
1.0 mL Bone Marrow Aspirate in a Lavender Top Tube - EDTA


TransportAndStorage

EDTA: 48 hours Ambient
Na Heparin: 48 hours Ambient


Collection Instructions

 


Minimum Volume


Neonatal Volumne

Clinical Interpretation

Reference Range:

Refer to Interpretive Pathology Flow Report

See CBC for its Reference Ranges


Test Comments:

The specimen is processed by whole blood lysis and stained with monoclonal antibodies using a direct immunofluorescence method. The cells are separated based on light scatter characteristics. and CD45 staining characteristics.

A Complete Blood Count will be reflexed, and charged with the ordering of this test if indicated


Methodology:
  • Flow Cytometry (FC)

Clinical Significance

Useful for distinguishing acute lymphoblastic leukemia from acute myelogenous leukemia and phenotypic subtyping of acute leukemias


Documentation

This test was developed and the performance characteristics determined by Parkview Health Laboratories. This test has not been cleared by the U.S. Food and Drug Administration. The FDA has determined that such clearance or approval is not necessary. This test is used for clinical purposes. It should not be regarded as investigational or for research. This laboratory is certified under the Clinical Laboratory Improvement Amendments of 1988 (CLIA-88) as qualified to perform high complexity clinical laboratory testing.


Production Schedule

Sites Performed
  • Parkview Regional Medical Center
Days Performed
Monday
Tuesday
Wednesday
Thursday
Friday
Saturday
Departments
  • Flow Cytometry
Turn Around Time

48 to 72 hours


Coding & Compliance

CDM

01399703, 01399705, 01430747


CPT Coding

85025, 88184, 88185 x 30 (Reflex 85007, 85008)