Antiphospholipid antibodies are found in a wide range of conditions, either transiently as in some infectious diseases, or more persistently in autoimmune diseases such as SLE. Antiphospholipid antibodies are associated with fetal loss, endocarditis, stroke, heart attack and autoimmune hemolytic anemia.
1.0 mL Serum in a SST Gold Top Tube
and
2.0 mL Plasma from two (2) Blue Top Tubes 3.2% Sodium Citrate in Plastic Vials
Serum
14 days Refrigerated
Plasma
14 days Frozen
1. First, Draw a plain red top tube to remove tissue fluid contamination.
2. Draw blood into a buffered citrate collection tube (light blue top) filled to proper level. Do not overfill.
3. The blood-to-anticoagulant ratio should be 9:1; inadequate filling of the collection device will decrease this ratio and
may lead to inaccurate results.
4. Invert gently 6 times to mix. Process immediately.
5. Centrifuge for 15 min at 2500 x g.
6. Remove plasma using a plastic pipette to transfer into a Plastic Vial.
7. Repeat centrifugation at 2500 x g for 15 minutes to assure complete platelet removal.
8. Dispense the plasma into 2 or more plastic tubes using a plastic transfer pipette. Label tubes appropriately.
9. Freeze immediately
10. Specimen must remain frozen at all times.
Antiphospholipid antibodies are found in a wide range of conditions, either transiently as in some infectious diseases, or more persistently in autoimmune diseases such as SLE. Antiphospholipid antibodies are associated with fetal loss, endocarditis, stroke, heart attack and autoimmune hemolytic anemia.
6 to 10 days
85613, 86146 x 3, 86147 x 3, 86148 x 2