BK Virus DNA, Quantitative Real-Time PCR, Plasma,Serum

Overview

  • EPIC Code:
  • LAB3240
  • Soft Test Code:
  • BKSE3
  • Send Out Test Code:
  • 11274
Alternate Names
  • BK Virus DNA, Quant PCR, BKV Polyoma, BK Virus DNA
  • BK Virus, Serum
  • BK Virus, Serum Quantitative
  • BKV
  • BKV, Serum
Clinical Significance

This test is used to determine the presence of BK virus DNA in patients' specimens.  Detection of the virus in these specimens may be indicative of an active infection, as PCR detects the presence of the virus, and not the hosts reaction to the virus.  Polyomavirus BK DNA detection in urine or plasma is associated with an increased risk of graft rejection in renal recipients.  Quantitative testing may indicate change in risk over time.  This is a quantitative molecular test, with a linear range of 500-500,000,000 copies/mL.


Specimen Collection & Preparation

Specimen Requirements

 0.7 mL plasma collected in an EDTA tube or ACD tube 
or
0.7 mL serum collected in a Serum Separator tube 
or 
0.7 mL whole blood collected in an EDTA tube or ACD tube

Alternate Specimen

 

TransportAndStorage

Plasma or serum:  transport frozen
Whole blood: transport refrigerated

Stability:
Plasma and serum
Ambient 48 hours
Refrigerated 7 days
Frozen 30 days

Whole blood
Ambient 48 hours
Refrigerated 8 days
Frozen unacceptable





Collection Instructions

Plasma:  collect blood in sterile tubes containing EDTA or in plasma preparation tube.  Collect whole blood at room temperature and separate plasma fom cells within 2 hours of collection.  Transfer plasma to sterile, plastic, screw-cap aliquot tubes.  Freeze and ship frozen.

Serum:  collect blood in sterile tubes with no anticoagulants;  Serum Separator Tubes (SST) are recommended.  Allow blood to clot at room temperature and separate serum within 1 hour of collection.  Transfer serum to sterile, plastic, screw-cap aliquot tubes.  Freeze and ship frozen.

Whole blood:  collect using standard procedures.  Store samples refrigerated following collection.  **DO NOT freeze whole blood.

Clinical Interpretation

Reference Range:

 Not detected


Methodology:
  • Real-Time Polymerase Chain Reaction

Clinical Significance

This test is used to determine the presence of BK virus DNA in patients' specimens.  Detection of the virus in these specimens may be indicative of an active infection, as PCR detects the presence of the virus, and not the hosts reaction to the virus.  Polyomavirus BK DNA detection in urine or plasma is associated with an increased risk of graft rejection in renal recipients.  Quantitative testing may indicate change in risk over time.  This is a quantitative molecular test, with a linear range of 500-500,000,000 copies/mL.


Production Schedule

Sites Performed
  • Quest - Chantilly
Days Performed
Sunday
Monday
Tuesday
Wednesday
Thursday
Friday
Saturday
Departments
  • Sendouts - Clinical
Turn Around Time

1 day


Coding & Compliance

CPT Coding

87799