BK Virus DNA, Quantitative, Plasma

Overview

  • EPIC Code:
  • LAB3240
  • Soft Test Code:
  • BKSE3
  • Send Out Test Code:
  • 11274
Alternate Names
  • BK Virus DNA, Quant PCR, BKV Polyoma, BK Virus DNA
  • BK Virus DNA, Quantitative Real-Time PCR, Plasma,Serum
  • BK Virus, Serum
  • BK Virus, Serum Quantitative
  • BKV
  • BKV, Serum
Clinical Significance

This test detects and quantitates BK Virus DNA in plasma specimens of kidney transplant recipients who are at risk of developing BKV-associated nephropathy (BKVAN) and associated hemorrhagic cystitis leading to kidney failure. Prospective viral load monitoring of BKV DNA in plasma (and urine) is useful to exclude a diagnosis of BKVAN and to monitor the course of BKV infection. The assay is calibrated to the First World Health Organization International Standard for BKV DNA with a Reportable Range of 21.5-100,000,000 IU/mL (1.33-8.00 log IU/mL).


Specimen Collection & Preparation

Specimen Requirements:

-OR-

2.0 mL plasma from a Lavendar Top Tube - EDTA in a Plastic Vial
Minimum Volume:
1.0 mL Plasma*

* This volume does not allow for repeat testing

Processing Instructions:

Centrifuge and separate from cells within 24 hours of collection. 


Rejection Criteria:

Green top Na heparin tube collection

Green top Li heparin tube collection

Unspun PPT tubes 

Frozen whole blood


Transport and Storage:
  • Ambient (18-24°C): Unacceptable
  • Refrigerated (2-8°C): 6 days

    Freshly drawn specimens (whole blood) may be stored at 2-25° C for up to 24 hours prior to centrifugation. 

  • Frozen (-20° C or colder): 6 months

Clinical Interpretation

Reference Range:

BK Virus DNA, QN PCR Not Detected (IU/mL)
BK Virus DNA, QN PCR Not Detected (Log IU/mL)


Methodology:
  • Real-Time Polymerase Chain Reaction

Clinical Significance

This test detects and quantitates BK Virus DNA in plasma specimens of kidney transplant recipients who are at risk of developing BKV-associated nephropathy (BKVAN) and associated hemorrhagic cystitis leading to kidney failure. Prospective viral load monitoring of BKV DNA in plasma (and urine) is useful to exclude a diagnosis of BKVAN and to monitor the course of BKV infection. The assay is calibrated to the First World Health Organization International Standard for BKV DNA with a Reportable Range of 21.5-100,000,000 IU/mL (1.33-8.00 log IU/mL).


Production Schedule

Sites Performed
  • Quest - Chantilly
Days Performed
Sunday
Monday
Tuesday
Wednesday
Thursday
Friday
Saturday
Departments
  • Sendouts - Clinical
Turn Around Time

1 day


Coding & Compliance

CDM

BK Virus DNA


CPT Coding

87799