Information regarding flow cytometry. The process involves a specimen-usually blood, by can be a piece of tissue like a lymph node, fluids, and bone marrow. The specimen is lysed to get rid of the red cells. Then it is added to specific antibodies that help the pathologist to determine if the patient has a lymphoma or leukemia. We also process peripheral blood to look for immunodeficiency, and HIV patients to check their T cell status.

 

The specifics:

 

CD4 (Tcell) and Lymph subset- are done on peripheral blood collected in a Sodium heparin (dark green with stripes on label) tube and kept at room temperature. These tests are batched and run on Monday, Wednesday, Friday, and Saturday

 

Acute Panels- are usually performed when blasts cells are present peripherally and/or a known leukemic patient for staging. This panel can be done on peripheral blood, but Bone Marrow is the preferred specimen.  Specimens should be collected into Sodium Heparin (Dark Green with stripes on label) tube and kept at room temperature. (Alternate specimen is Lavender tube at room temperature)

 

B-cell Clonal Panel - Peripheral blood or Bone Marrow can be used. Collect in Sodium Heparin (Dark Green with stripes) and kept at room temperature. (Alternate specimen is Lavender tube at room temperature)

 

Tissue- This is ordered by the pathologist for a tissue sample, like a lymph node or mass that has been collected in surgery. These specimens are put in RPMI, a culture media, to preserve it until testing is done. These samples are kept refrigerated until tested.

 

Fluids- No Additive Tube or EDTA (lavender) tube. Fluids should be refrigerated until tested. Complete panels can be done on fluids.

 

Bronchial lavage fluids- CD4/CD8 ratio: All BAL specimens will automatically reflex CD4/CD8 ratio when the lymphocyte count is > 15%.

 

**Any other requests- Contact the Client Response Center  (630am - 300pm).

LAB SYSTEM ORDER CODE

EPIC ORDER EAP

EPIC PROCEDURE NAME

SOFT test REPORTED TO EPIC

EPIC RESULT LRR

TESTING

NOTES

FACUT- peripheral blood (reflexes CBCWD)

LAB2452

ACUTE LEUKEMIA PANEL(FACUT)

ACUTE LEUKEMIA PANEL

FACUT

Aid in identifying Blasts in BM or peripheral

 

FBCLL- peripheral blood (reflexes CBCWD)

LAB2453

B-CELL (CLL) PANEL(FBCLL)

B-CELL CLONAL PANEL

FBCLL

To rule out CLL or Lymphoma (lymphocyte disorder)

 

FLFLO- Body fluid sepcimen (see below for BAL specimens)

LAB6946

FLOW CYTOMETRY FLUID SPECIMEN

FLOW CYTOMETRY fluid

FLFLO

CSF fluids-ex: pleural, BAL

FACUT, FBCLL, FLFLO, FLYMP, FS: For results, See Pathology Dept. Interpretation

FLHSR this is a reflexed test for BAL specimens

LAB2944

CD4:CD8 Ratio; Bronchial Lavage

 

FLHSR

FCELL needs ordered to reflex CD4/CD8 Ratio done when lymphocyte count is ≥15%

FLYMP - tissue samples

LAB2457

FLOW CYTOMETRY LYMPHOMA(FLYMP)

FLOW CYTOMETRY LYMPHOMA

FLYMP

For tissue bx samples. For Blood: order FBCLL

FS - reflexes CBC 

LAB2454

FLOW CYTOMETRY SPECIMEN(FS)

FLOW CYTOMETRY SPECIMEN

FS

When not sure what to order. not comment what you want 

FTCEL- CD4, Tcells (HIV monitoring) (reflexes CBC)

LAB342

T-HELPER CELLS (CD4) COUNT(FTCEL)

CD3 (MATURE T)%

CD3%

 

FTCEL, FLSNK: will be resulted with values into EPIC.

CD3 (MATURE T),Abs

CD3#

CD4+/CD3+(HELPER)%

CD4%

CD4+/CD3+(HELPER),Abs

CD4#

CD8+/CD3+(SUPP)%

CD8%

CD8+/CD3+(SUPP),Abs

CD8#

HELPER/SUPP RATIO

HSRAT

Lymph Subset (Reflexes CBCWD) 

LAB2458

LYMPH SUBSET Panel

CD3 (MATURE T)%

CD3%

 

CD3 (MATURE T),Abs

CD3#

CD4+/CD3+(HELPER)%

CD4%

CD4+/CD3+(HELPER), abs

CD4#

CD8+/CD3+(SUPP)%

CD8%

CD8+/CD3+(SUPP),Abs

CD8#

CD19 (PAN B)%

CD19P

CD19 (PAN B),Abs

CD19A

CD56+/CD3-(NK) %

CD56P

CD56+/CD3-(NK),Abs

CD56A

HELPER/SUPP RATIO

HSRAT

 

  
 

 

 

 

 

 
CDMS  LAB3046   CD19 & CD20  CD19% & CD20%    
CD19  LAB3047 CD19  CD19%    
CD20  LAB3048  CD20  CD20%      
FDNA  LAB2455 Flow Cytometry DNA (FDNA)  Flow Cytometry DNA FDNA Do not use, testing ordered by Pathologist