May 31, 2016
New In-House Molecular Tests for GI Pathogens and Meningitis/Encephalitis
The Microbiology Department is now running two new molecular panels:
Epic Name: Gastrointestinal Pathogen Panel by BioFire PCR
Specimen: Stool placed in Cary Blair Vial (green top stool vial—HEMM #91915) within two hours. No endoscopy stool aspirates or rectal swabs.
TAT: Within 24 hours after receipt in the Microbiology Lab
Enteric Pathogens detected by PCR:
Bacteria: Campylobacter (C. jejuni/C. coli/C. upsaliensis), Clostridium difficile toxin A/B, Plesiomonas shigelloides, Salmonella, Vibrio (V. parahaemolyticus/V.vulnificus/V. cholerae), Yersinia enterocolitica, Diarrheagenic E. coli (Enteroaggregative E. coli (EAEC), Enteropathogenic E. coli (EPEC), Enterotoxigenic (ETEC) heat-labile (lt) and heat-stable (st) Enterotoxins , Shiga-like toxin-producing E. coli (STEC) including E. coli O157, Shigella/Enteroinvasive E. coli (EIEC).
Viruses: Adenovirus F 40/41, Astrovirus, Norovirus GI/GII, Rotavirus A, Sapovirus (Genogroups I, II, IV and V).
Parasites: Cryptosporidium, Cyclospora cayetanensis, Entamoeba histolytica, Giardia lamblia
Epic Name: Meningitis Encephalitis Pathogen Panel by BioFire PCR
Specimen: CSF from tap (no shunt fluid)
TAT: Within 4 hours after receipt in the Microbiology Lab
Meningitis/Encephalitis Pathogens detected by PCR:
Bacteria: Escherichia coli, Haemophilus influenzae, Listeria monocytogenes, Neisseria meningitidis, Streptococcus agalactiae, Streptococcus pneumoniae
Viruses: Cytomegalovirus (CMV), Enterovirus, Herpes simplex virus 1 (HSV 1), Herpes simplex virus 2 (HSV 2), Human herpes virus 6 (HHV 6), Parechovirus, Varicella-zoster virus (VZV)
Fungi: Cryptococcus neoformans/gattii
May 23, 2016
Parkview Health Laboratories presently reflexes a Group A Strep Culture on all negative Rapid Strep A results. Beginning May 31st we will begin reflexing cultures on only those patients less than eighteen years of age.
May 19, 2016
For those patients eighteen and older, a Group A Strep Culture (Soft Code: CXGRA) or Throat Culture (Soft Code: CXTHR) may be ordered separately if desired.
On June 1, 2016, PRMC Chemistry will begin Procalcitonin (PCT) testing. The specimen requirement will be 1 ml plasma from Lithium Heparin (Mint Green top PST) tube. Testing will be performed Monday-Sunday on all shifts.
The reference ranges are:
PCT <=0.5 ng/ml = Low risk for progression to severe systemic infection(severe sepsis/septic shock.
CAUTION: PCT levels below 0.5 ng/ml do not exclude infection, because localized infections (without systemic signs) may be associated with such low levels. If PCT is measured very early after a bacterial challenge (usually <6 hours), these values may still be low. In this case, PCT should be re-assessed 6-24 hours later.
PCT >0.5 to <=2 ng/ml= Moderate risk for progression to severe systemic infection (severe sepsis/septic shock).
The patient should be closely monitored both clinically and by re-assessing PCT within 6-24 hours.
PCT >2 ng/ml = High risk for progression to severe systemic infection (severe sepsis/septic shock).
For questions, contact Janet Benoit, Laboratory Technical Manager, at 266-1505, Richard Brown, Laboratory Chemistry Specialist, at 266-1521, or Dr. Steven Wang, Laboratory Pathologist, at 266-1640.
May 6, 2016
Changes effective 5-17-16, Prothrombin time reference range 9.4-11.6 seconds, APTT reference range 23.8-33.8 seconds, INR upper reportable range >8.9. If you have any questions please contact Dr. Cindy Nie at 266-1641.
On May 17, 2016, the CK and reflex CKMB will be removed from the Cardiac Stat Panel (test code CARDC). The CK or the CKMB (which includes CK & CKMB) can be ordered as needed. For questions, contact Janet Benoit, Laboratory Technical Manager, at 266-1505, Richard Brown, Laboratory Chemistry Specialist, at 266-1521, or Dr Steven Wang, Laboratory Pathologist, at 266-1640.