Flow Cytometry Orders
Information regarding flow cytometry. The process involves a specimen-usually blood, by can be a piece of tissue like a lymph node, fluids, and bone marrow. The specimen is lysed to get rid of the red cells. Then it is added to specific antibodies that help the pathologist to determine if the patient has a lymphoma or leukemia. We also process peripheral blood to look for immunodeficiency, and HIV patients to check their T cell status.
So, the specifics:
CD4 (Tcell) and Lymph subset- are done on peripheral blood collected in a Sodium heparin (dark green with stripes on label) tube and kept at room temperature. These tests are batched and run on Monday, Wednesday, Friday, and Saturday
Acute Panels- are usually performed when blasts cells are present peripherally and/or a known leukemic patient for staging. This panel can be done on peripheral blood, but Bone Marrow is the preferred specimen. Specimens should be collected into Sodium Heparin (Dark Green with stripes on label) tube and kept at room temperature. (Alternate specimen is Lavender tube at room temperature)
B-cell Clonal Panel - Peripheral blood or Bone Marrow can be used. Collect in Sodium Heparin (Dark Green with stripes) and kept at room temperature. (Alternate specimen is Lavender tube at room temperature)
Tissue- This is ordered by the pathologist for a tissue sample, like a lymph node or mass that has been collected in surgery. These specimens are put in RPMI, a culture media, to preserve it until testing is done. These samples are kept refrigerated until tested.
Fluids- No Additive Tube or EDTA (lavender) tube. Fluids should be refrigerated until tested. Complete panels can be done on fluids.
Bronchial lavage fluids- CD4/CD8 ratio: All BAL specimens will automatically reflex CD4/CD8 ratio when the lymphocyte count is > 15%.
LAB SYSTEM ORDER CODE
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EPIC ORDER EAP
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EPIC PROCEDURE NAME
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SOFT test REPORTED TO EPIC
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EPIC RESULT LRR
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TESTING
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NOTES
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FACUT- peripheral blood (reflexes CBCWD)
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LAB2452
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ACUTE LEUKEMIA PANEL(FACUT)
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ACUTE LEUKEMIA PANEL
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FACUT
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Aid in identifying Blasts in BM or peripheral
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FBCLL- peripheral blood (reflexes CBCWD)
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LAB2453
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B-CELL (CLL) PANEL(FBCLL)
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B-CELL CLONAL PANEL
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FBCLL
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To rule out CLL or Lymphoma (lymphocyte disorder)
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FLFLO- Body fluid sepcimen (see below for BAL specimens)
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LAB6946
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FLOW CYTOMETRY FLUID SPECIMEN
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FLOW CYTOMETRY fluid
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FLFLO
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CSF
fluids-ex: pleural, BAL
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FACUT, FBCLL, FLFLO, FLYMP, FS: For results, See Pathology Dept. Interpretation
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FLHSR this is a reflexed test for BAL specimens
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LAB2944
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CD4:CD8 Ratio; Bronchial Lavage
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FLHSR
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FCELL needs ordered to reflex CD4/CD8 Ratio done when lymphocyte count is ≥15%
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FLYMP - tissue samples
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LAB2457
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FLOW CYTOMETRY LYMPHOMA(FLYMP)
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FLOW CYTOMETRY LYMPHOMA
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FLYMP
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For tissue bx samples. For Blood: order FBCLL
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FS - reflexes CBC
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LAB2454
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FLOW CYTOMETRY SPECIMEN(FS)
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FLOW CYTOMETRY SPECIMEN
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FS
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When not sure what to order. not comment what you want
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FTCEL- CD4, Tcells (HIV monitoring) (reflexes CBC)
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LAB342
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T-HELPER CELLS (CD4) COUNT(FTCEL)
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CD3 (MATURE T)%
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CD3%
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FTCEL, FLSNK: will be resulted with values into EPIC.
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CD3 (MATURE T),Abs
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CD3#
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CD4+/CD3+(HELPER)%
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CD4%
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CD4+/CD3+(HELPER),Abs
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CD4#
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CD8+/CD3+(SUPP)%
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CD8%
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CD8+/CD3+(SUPP),Abs
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CD8#
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HELPER/SUPP RATIO
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HSRAT
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Lymph Subset (Reflexes CBCWD)
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LAB2458
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LYMPH SUBSET Panel
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CD3 (MATURE T)%
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CD3%
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CD3 (MATURE T),Abs
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CD3#
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CD4+/CD3+(HELPER)%
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CD4%
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CD4+/CD3+(HELPER), abs
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CD4#
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CD8+/CD3+(SUPP)%
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CD8%
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CD8+/CD3+(SUPP),Abs
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CD8#
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CD19 (PAN B)%
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CD19P
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CD19 (PAN B),Abs
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CD19A
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CD56+/CD3-(NK) %
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CD56P
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CD56+/CD3-(NK),Abs
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CD56A
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HELPER/SUPP RATIO
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HSRAT
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CDMS |
LAB3046 |
CD19 & CD20 |
CD19% & CD20% |
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CD19 |
LAB3047 |
CD19 |
CD19% |
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CD20 |
LAB3048 |
CD20 |
CD20% |
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FDNA |
LAB2455 |
Flow Cytometry DNA (FDNA) |
Flow Cytometry DNA |
FDNA |
Do not use, testing ordered by Pathologist |
**Any other requests- Contact Laboratory Client Response Center (630am - 1500).