Information regarding flow cytometry. The process involves a specimen-usually blood, but can be a piece of tissue like a lymph node, fluids, and bone marrow.    The specimen is lysed to get rid of all the red cells-we have many. Then it is added to specific antibodies that help the pathologist to determine if the patient has a lymphoma or leukemia. We also process peripheral blood for HIV patients to check their T cell status and for allergy patients to see if their lymphs are stimulated.

So, the specifics—
Tests—CD4, Tcell, Lymph subsets and NK panels are usually done on peripheral blood collected in a Sodium Heparin (Dark Green with stripes)  tube and left at room temperature. These tests are batched and run on Monday, Wednesday, and Friday.
Acute panels-are usually performed when Blast cells are present peripherally and /or a known leukemic patient for staging. This panel can be done on peripheral blood, but BONE Marrow is the preferred specimen. Again, both specimens should be collected in a Sodium Heparin (Dark Green with stripes) tube and left at room temperature.
CLL/Lymphoma panels-are usually performed when the Lymphocyte counts are high. Again, peripheral blood or Bone Marrow can be used—collected in Sodium Heparin (Dark Green with stripes) and left at room temperature.
Lymphoma panel—this is ordered by the pathologist for a tissue sample-like a lymph node or mass that has been collected in surgery. These specimens are put in RPMI-a cell culture media to preserve it until testing is done. These samples are kept refrigerated until tested.
Fluids can be run for flow cytometry also. They can be in a Sodium Heparin (Dark Green with stripes) tube or EDTA (lavender) tube. Fluids should be refrigerated until tested. Complete panels can be done on fluids. Lately, BALs have been ordered for CD4/CD8 ratio.
DNAs-We can also perform testing on cell blocks from Histology, but these are ordered by a pathologist. They are very labor-intensive and require at least 3 days to complete.

LAB SYSTEM ORDER CODE EPIC ORDER EAP EPIC PROCEDURE NAME SOFT test REPORTED TO EPIC EPIC RESULT LRR Testing NOTES
FACUT- peripheral blood, reflexes CBC also LAB2452 ACUTE LEUKEMIA PANEL(FACUT) ACUTE LEUKEMIA PANEL FACUT Aid in identifying Blasts in BM or peripheral FACUT, FBCLL, FLFLO, FLYMP, FS: no results will be entered for these tests. See Pathology Interpretation for results.
FBCLL- peripheral blood, reflexes CBC also LAB2453 B-CELL (CLL) PANEL(FBCLL) B-CELL CLONAL PANEL FBCLL To rule out CLL or Lymphoma (lymphocyte disorder)
FLFLO: for fluids such as pleural, BAL LAB6946 FLOW CYTOMETRY FLUID SPECIMEN FLOW CYTOMETRY fluid FLFLO fluids-ex: pleural, BAL
FLYMP: for tissue samples LAB2457 FLOW CYTOMETRY LYMPHOMA(FLYMP) FLOW CYTOMETRY LYMPHOMA FLYMP Ordered by Lab, for tissue bx. For Blood: order FBCLL
FS - reflexes CBC also LAB2454 FLOW CYTOMETRY SPECIMEN(FS) FLOW CYTOMETRY SPECIMEN FS option- when do not know what to order. But Lab will need to have copy of order.
FDNA LAB2455 FLOW CYTOMETRY DNA(FDNA) FLOW CYTOMETRY DNA FDNA do not use do not use
FSDNA LAB2456 FLOW CYTOMETRY S- PHASE DNA(FSDNA) FLOW CYTOMETRY S- PHASE DNA FSDNA do not use do not use
FLSUB - T and B cells. (reflexes CBC also) LAB2458 LYMPH SUBSET                  (T and B cells) (FLSUB)     (not NK cells) CD2(PAN T)% CD2% (Tcells) FLSUB, FTCEL, FLSNK: will be resulted with values into EPIC.
CD2(PAN T) CD2#
CD3 (MATURE T)% CD3%
CD3 (MATURE T) CD3#
CD4+/CD3+(HELPER)% CD4%
CD4+/CD3+(HELPER) CD4#
CD8+/CD3+(SUPP) CD8%
CD8+/CD3+(SUPP) CD8#
CD19 (PAN B)% CD19P (Bcells)
CD19 (PAN B) CD19A
HELPER/SUPP RATIO HRSAT  
LYMPHOCYTE SUBSET LSCOM  
FTCEL- CD4, Tcells (HIV monitoring). (reflexes CBC also) LAB342 T-HELPER CELLS (CD4) COUNT(FTCEL) CD3 (MATURE T)% CD3%  
CD3 (MATURE T) CD3#  
CD4+/CD3+(HELPER)% CD4%  
CD4+/CD3+(HELPER) CD4#  
CD8+/CD3+(SUPP) CD8%  
CD8+/CD3+(SUPP) CD8#  
HELPER/SUPP RATIO HSRAT  
FLSNK- T and B and NK cells. (reflexes CBC also) LAB2462 LYMPH SUBSET WITH NK CELLS(FLSNK) Bitmap
CD2(PAN T)%
CD2%  
CD2(PAN T) CD2#  
CD3 (MATURE T)% CD3%  
CD3 (MATURE T) CD3#  
CD4+/CD3+(HELPER)% CD4%  
CD4+/CD3+(HELPER) CD4#  
CD8+/CD3+(SUPP) CD8%  
CD8+/CD3+(SUPP) CD8#  
CD19 (PAN B)% CD19P  
CD19 (PAN B) CD19A  
CD16+/CD3-(NK) % CD16P (Nkcells- killer)
CD16+/CD3-(NK) CD16A
CD56+/CD3-(NK) % CD56P
CD56+/CD3-(NK) CD56A
HELPER/SUPP RATIO HSRAT  
LYMPHOCYTE SUBSET LSCOM  
**Any other requests- Contact Flow dept (630am - 1500). 
**Lab: for Special requests (such as BAL CD4:CD8, or CD19, CD20) when flow is closed, please order MFLO and provide req for flow to interpret and order.