Keep acid tube cold before collection
Collect 4.0 mL Whole Blood in a Lavender Top Tube - EDTA
Immediately vigorously mix with 4.0 mL prechilled, ice cold 7% or 8% perchloric acid
Let mixture stand for 10 minutes
Centrifuge and separate supernatant fluid.
Separate and submit 4.5 mL supernant fluid for assay refigerate
Maintain 1:1 ratio for whole blood and Perchloric acid
Order must include volumes of blood and perchloric acid used.