Last Modified:
9/22/2021 2:46:21 PM
Medical Necessity Documentation:
Client Notes:
Patient Preparation:
Specimen Requirements:
1.0 mL Frozen Plasma from a Blue Top Tube - 3.2% Na Citrate in a Plastic Vial
Collection Instructions:
Centrifuge light blue-top tube for 15 minutes at approximately 1500 g within 60 minutes of collection. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial. Centrifuge a second time and transfer platelet-poor plasma into a new plastic vial. Plasma must be free of platelets (<10,000/mcL).
Minimum Volume:
0.5 mL platelet poor plasma
Transport & Storage: Temperature/Stability:
Unacceptable Ambient
Unacceptable Refrigerated
30 days Frozen -20°C
Freeze immediately and ship on dry ice.
Note: Storage of whole blood at refrigerated temperatures prior to processing may lead to cryoprecipitate formation and falsely low Factor VIII and von Willebrand Factor studies.
Reference Range:
50% - 180% of normal
Critical Ranges:
Test Comments:
For patients receiving Hemilbra (aka emicizumab), the Factor VIII activity clotting method is not recommended. Hemilbra will cause falsely elevated Factor VIII activity values. A chromogenic Factor VIII Activity method is recommended.
Methodology:
Clotting Detection
Clinical Significance:
The most common form of hemophilia, Hemophilia A, is caused by a deficiency of Factor VIII. Hemophilia A is an X-linked disorder affecting between 1 in 5000 to 10000 males. Measurement of Factor VIII activity levels is used to classify Hemophilia A (mild, moderate or severe) and to assess response to treatment.
Documentation:
Custom Panel:
No
PRODUCTION SCHEDULE
Turn Around Time:
3 to 5 days
Days Performed:
Monday, Tuesday, Wednesday, Thursday, Friday
Sites Performed:
Quest - Chantilly
PHL Test Code:
FACT8
EPIC Test Code:
LAB306
Send Out Test Code:
347
Alternate Test Names:
Anti-Hemophilia; Factor 8; Factor VIII Assay; Factor VIII Functional; Hemophilia A; Procoagulant Factor 8
Included Tests:
CPT Coding:
85240
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