The most common form of hemophilia, Hemophilia A, is caused by a deficiency of Factor VIII. Hemophilia A is an X-linked disorder affecting between 1 in 5000 to 10000 males. Measurement of Factor VIII activity levels is used to classify Hemophilia A (mild, moderate or severe) and to assess response to treatment.
1.0 mL Frozen Plasma from a Blue Top Tube - 3.2% Na Citrate in a Plastic Vial
Unacceptable Ambient
Unacceptable Refrigerated
30 days Frozen -20°C
Freeze immediately and ship on dry ice.
Note: Storage of whole blood at refrigerated temperatures prior to processing may lead to cryoprecipitate formation and falsely low Factor VIII and von Willebrand Factor studies.
Centrifuge light blue-top tube for 15 minutes at approximately 1500 g within 60 minutes of collection. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial. Centrifuge a second time and transfer platelet-poor plasma into a new plastic vial. Plasma must be free of platelets (<10,000/mcL).
0.5 mL platelet poor plasma
50% - 180% of normal
For patients receiving Hemilbra (aka emicizumab), the Factor VIII activity clotting method is not recommended. Hemilbra will cause falsely elevated Factor VIII activity values. A chromogenic Factor VIII Activity method is recommended.
The most common form of hemophilia, Hemophilia A, is caused by a deficiency of Factor VIII. Hemophilia A is an X-linked disorder affecting between 1 in 5000 to 10000 males. Measurement of Factor VIII activity levels is used to classify Hemophilia A (mild, moderate or severe) and to assess response to treatment.
3 to 5 days
01453628
85240