Last Modified:
2/2/2023 12:02:03 PM
Medical Necessity Documentation:
Due to reagent backorder, Serotonin Release Assay for Unfractionated Heparin will be temporarily sent to Mayo Clinic Laboratories. Serotonin Release Assay for Low Molecular Weight Heparin is temporarily unavailable. Please contact the Lab Sendout department at 266-1600 with questions about this temporary testing change
Client Notes:
Patient Preparation:
Specimen Requirements:
2.0 mL Serum from a SST Gold Top Tube
Collection Instructions:
Specimen should be drawn by venipuncture. Do not collect specimen from a central line.
Minimum Volume:
1.0 mL Serum
Transport & Storage: Temperature/Stability:
48 hours Refrigerated
Rejection Criteria:
Lithium Heparin plasma
Reference Range:
Negative
Critical Ranges:
Test Comments:
Although a negative Platelet Factor 4 (PF4) result (OD <0.399) does not totally exclude a diagnosis of Heparin Induced Thrombocytopenia (HIT), this test is about 95% sensitive for detecting antibodies to the PF4-Heparin complex. No treatment decisions should be made based solely on this lab result, but should be made in conjunction with other laboratory findings and correlation with the patient's clinical condition.
Not uncommonly, heparin therapy induces Platelet Factor 4 (PF4) antibody without clinical heparin induced thrombocytopenia (HIT). A positive PF4 result does not confirm a diagnosis of HIT. While a strong positive value (OD >1.000) increases the likelihood of HIT, a functional assay, such as the Serotonin Release Assay (SRA) is more specific and is recommended to assist with the diagnosis, if clinically warranted. No treatment decisions should be made based solely on this lab result, but should be made in conjunction with other laboratory findings and correlation with the patient's clinical condition.
Methodology:
EIA
Clinical Significance:
Documentation:
Custom Panel:
No
PRODUCTION SCHEDULE
Days Performed:
Sunday, Monday, Tuesday, Wednesday, Thursday, Friday, Saturday
Sites Performed:
Parkview Regional Medical Center, Quest - Chantilly
PHL Test Code:
PF4R
EPIC Test Code:
LAB3173
Alternate Test Names:
Heparin Induced Platelet Antibody ; Heparin Induced Thrombocytopenia; HIT; PF4; PF4 with Reflex
Included Tests:
CPT Coding:
86022 Reflex 86022 x 2
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