Medical Necessity Documentation:
Client Notes:
Patient Preparation:
12 hour Fasting preferred, but not required.
Patient should discontinue taking Biotin, Vitamin B7, or Vitamin H 48 hours before getting blood drawn.
Specimen Requirements:
1.0 mL Serum in a SST Gold Top Tube
Collection Instructions:
Minimum Volume:
0.3 mL Serum
Transport & Storage: Temperature/Stability:
4 days Refrigerated
24 weeks Frozen
Avoid storage in frost free freezers to avoid repeated freeze/thaw cycles
Rejection Criteria:
Plasma from/in a PST Mint Green Top Tube - Li Heparin
Reference Range:
30-100 ng/mL
Critical Ranges:
Test Comments:
Values <30 ng/mL: 25-OH Vitamin D levels below 20 ng/mL are indicative of a deficiency. Levels between 20 and 30 ng/mL are indicative of an insufficiency.
Patients taking vitamin supplements containing high doses of biotin (>5 mg/day) may have falsely increased test results generated
Methodology:
EIA
Clinical Significance:
Vitamin D is obtained from food and supplements or made in the skin by exposure to sunlight. 25-OH Vitamin D is formed from these Vitamin D sources and stored in the liver. 25-OH Vitamin D is then changed to its active form, 1,25-dihydroxy Vitamin D, in the kidney. 1,25-dihydroxy Vitamin D keeps normal levels of calcium and phosphate in the blood through its actions on the intestines, kidneys, and bones.
The most reliable clinical indicator of Vitamin D status is 25-OH Vitamin D because serum or plasma levels reflect the body’s storage levels of Vitamin D. 25-OH Vitamin D levels correlate with the clinical symptoms of Vitamin D deficiency.
Abnormal 1,25-dihydroxy Vitamin D levels may be seen in primary hyperparathyroidism, physiologic hyperparathyroidism secondary to low calcium or vitamin D intake, hypoparathyroidism, and chronic renal failure. 1,25-dihydroxy Vitamin D levels may be needed to adequately assess Vitamin D status in the presence of renal disease.
25-OH Vitamin D level is performed by PHL and should be ordered as test code VD251. 1,25-dihydroxy Vitamin D is performed by Quest Diagnostics and should be ordered as test code VITD2.
Documentation:
Custom Panel:
No
PRODUCTION SCHEDULE
Days Performed:
Sunday, Monday, Tuesday, Wednesday, Thursday, Friday, Saturday
Sites Performed:
Parkview Regional Medical Center
PHL Test Code:
DAVD
EPIC Test Code:
LAB3277
Alternate Test Names:
25 Hydroxy Vitamin D; 25HDN; 25-Hydroxy D2; 25-Hydroxy D3; 25-Hydroxy Vitamin D; 25-Hydroxycholecalciferol; 25-Hydroxycholecalciferol (25OHD3); 25-Hydroxyergocalciferol; 25-Hydroxyergocalciferol (25OHD2); 25-Hydroxyvitamin D2; 25-Hydroxyvitamin D3; 25ODH; 25-OH D2; 25-OH D3; 25-OH-D2; 25-OH-D3; Bit D 25 Hydrox Direct Access; Calcidiol; Calcifidiol; Cholecalciferol; Colecalciferol; D2 ; D2 Vitamin; D3 ; D3 Vitamin; Ergocalciferol; Vit D; Vit D 25 Hydrox Direct Access; Vit D 25 OH; Vitamin D; Vitamin D, 25 OH; Vitamin D, 25 OH Total, D2, D3; Vitamin D, 25-Hydroxy; Vitamin D, 25-OH; Vitamin D, Total; Vitamin D2; Vitamin D2, 25-hydroxy; Vitamin D3; Vitamin D3, 25-hydroxy
Included Tests:
CPT Coding:
82306