Lymphocyte Subset Panel with Natural Killer Cells

Last Modified: 5/17/2019 2:24:50 PM


Medical Necessity Documentation:  
Client Notes:  
Patient Preparation:  
Specimen Requirements:

4.0 mL Whole Blood in a Green Top Tube - Na Heparin 
and
3.0 mL Whole Blood in a Lavender Top Tube - EDTA

Collection Instructions:  
Transport & Storage: Temperature/Stability: 48 hours Ambient - Na Heparin
24 hours Ambient - EDTA
Reference Range:
Pediatric (less than 3 years)
CD2 (Pan T-cells): 55% - 88% / 2200 - 3500 cells/uL
CD3 (Mature T-cells): 55% - 82% / 2324 - 3295 cells/uL
CD4+/CD3+ (Helper T-cells): 38% - 46% / 1538 - 2213 cells/uL
CD8+/CD3+ (Suppressor T-cells): 8% - 31% / 351 - 2479 cells/uL
CD4 / CD8 Ratio: 1.2 - 6.2
CD16+ / CD3- (NK Cells): 0% - 16% / 0 - 1200 cells/uL
CD19 (B-cells): 11% - 45% / 430 - 3300 cells/uL
CD 56 / CD3- (NK Cells): 0% - 16% / 0 - 1200 cells/uL

Adult
CD2 (Pan T-cells): 68% - 94% / 500 - 2900 cells/uL
CD3 (Mature T-cells): 61% - 91% / 558 - 2061cells/uL
CD4 (Helper T-cells): 32% - 65% / 487 - 1678 cells/uL
CD8 (Suppressor T-Cells): 13% - 39% / 201 - 1045 cells/uL
CD4/CD8 Ratio: 0.9 - 3.7
CD16+ / CD3- (NK Cells): 1% - 29% / 0 - 800 cells/uL
CD19 (B-cells): 4% - 25% / 30 - 550 cells/uL
CD 56 / CD3- (NK Cells): 1% - 29% / 0 - 800 cells/uL


See CBC for its Reference Ranges
Critical Ranges:  
Test Comments:  
Methodology: Flow Cytometry
Clinical Significance:  
Documentation:
This test was developed and the performance characteristics determined by Parkview Health Laboratories. This test has not been cleared by the U.S. Food and Drug Administration. The FDA has determined that such clearance or approval is not necessary. This test is used for clinical purposes. It should not be regarded as investigational or for research. This laboratory is certified under the Clinical Laboratory Improvement Amendments of 1988 (CLIA-88) as qualified to perform high complexity clinical laboratory testing.
Custom Panel: No

PRODUCTION SCHEDULE

Turn Around Time: 24 to 72 hours
Days Performed: Monday, Wednesday, Friday
Sites Performed: Parkview Regional Medical Center, Parkview Wabash, Parkview Warsaw
PHL Test Code: FLSNK
EPIC Test Code: LAB2462
Alternate Test Names: Flow Cytometry Lymph Subset NK Cells; Lymph Subset with Natural Killer Cells; Lymph Subset with NK Cells; Natural Killer T Cell Count; T Cells; TCells; T-Cells
Included Tests: CD2; CD3; CD4; CD8; CD16; CD19; CD56; CD4/CD8 (Helper/Suppressor Ratio); CBC [Hematocrit (Hct); Hemoglobin (Hgb); Mean Corpuscular Hemoglobin (MCH); Mean Corpuscular Hemoglobin Concentration (MCHC); Mean Corpuscular Volume (MCV); Platelet (PLT);  Red Blood Cell Count (RBC); Red Cell Distribution Width Standard Deviation (RDWSD);  Red Cell Distribution Width Coefficient Variation (RDWCV); White Blood Cell Count (WBC)]
CPT Coding: 86355, 86356, 86357, 86360

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