Parkview Health Laboratory: Test Directory

Specimen Requirements:

4.0 mL Whole Blood in a Green Top Tube - Na Heparin
and
3.0 mL Whole Blood in a Lavender Top Tube - EDTA

Collection Instructions:

Collect Monday through Friday Only

Transport & Storage: Temperature/Stability: 24 to 48 hours Ambient

Reference Range:

Pediatric (less than 3 years)
CD2 (Pan T-cells): 55% - 88% / 2200 - 3500 cells/uL
CD3 (Mature T-cells): 55% - 82% / 2324 - 3295 cells/uL
CD4+/CD3+ (Helper T-cells): 38% - 46% / 1538 - 2213 cells/uL
CD8+/CD3+ (Suppressor T-cells): 8% - 31% / 351 - 2479 cells/uL
CD4 / CD8 Ratio: 1.2 - 6.2
CD16+ / CD3- (NK Cells): 0% - 16% / 0 - 1200 cells/uL
CD19 (B-cells): 11% - 45% / 430 - 3300 cells/uL
CD 56 / CD3- (NK Cells): 0% - 16% / 0 - 1200 cells/uL

Adult

CD2 (Pan T-cells): 68% - 94% / 500 - 2900 cells/uL
CD3 (Mature T-cells): 61% - 91% / 558 - 2061cells/uL
CD4 (Helper T-cells): 32% - 65% / 487 - 1678 cells/uL

CD8 (Suppressor T-Cells): 13% - 39% / 201 - 1045 cells/uL

CD4/CD8 Ratio: 0.9 - 3.7
CD16+ / CD3- (NK Cells): 1% - 29% / 0 - 800 cells/uL
CD19 (B-cells): 4% - 25% / 30 - 550 cells/uL
CD 56 / CD3- (NK Cells): 1% - 29% / 0 - 800 cells/uL

CBC:

For Pediatric Reference Ranges refer to Addendum B
Males > 14 Yrs

WBC: 4.5 - 11.0 TH/UL

RBC: 4.3 - 5.7 MI/UL

Hgb: 13.5 - 17.2 G/DL

Hct: 40.0 - 54.0%

MCV: 82.0 - 98.0 FL

MCH: 27.0 - 33.0 PG

MCHC: 32.0 - 36.0 G/DL

Platelet: 150.0 - 450.0 TH/UL

RDWSD: 36.4 - 54.5 FL

RDWCV: 11.5 - 14.5%

Females > 14 Yrs

WBC: 4.5 - 11.0 TH/UL

RBC: 4.0 - 5.2 MI/UL

Hgb: 12.0 - 15.5 G/DL

Hct: 35.0 - 48.0%

MCV: 82.0 - 98.0 FL

MCH: 27.0 - 33.0 PG

MCHC: 32.0 - 36.0 G/DL

Platelet: 150.0 - 450.0 TH/UL

RDWSD: 36.4 - 54.5 FL

RDWCV: 11.5 - 14.5%

Methodology: Flow Cytometry

Documentation:

The CDC Guidelines for the performance of CD4+ T-Cell Determinations in persons with Human Immunodeficiency Virus Infection recommends the Lymphocyte Subset Panel to achieve baseline quantitations for T & B cell populations. MMWR Vol 41/No. RR-8, 1992.

Custom Panel: No

Turn Around Time: 24 to 72 hours

Days Performed: Monday, Wednesday, Friday

Sites Performed: Parkview Regional Medical Center

PHL Test Code: FLSNK

Alternate Test Names: Flow Cytometry Lymph Subset NK Cells; Lymph Subset with Natural Killer Cells; Lymph Subset with NK Cells; T Cells; TCells; T-Cells

Included Tests: CD2; CD3; CD4; CD8; CD16; CD19; CD56; CD4/CD8 (Helper/Suppressor Ratio); CBC [Hematocrit (Hct); Hemoglobin (Hgb); Mean Corpuscular Hemoglobin (MCH); Mean Corpuscular Hemoglobin Concentration (MCHC); Mean Corpuscular Volume (MCV); Platelet (PLT); Red Blood Cell Count (RBC); Red Cell Distribution Width Standard Deviation (RDWSD); Red Cell Distribution Width Coefficient Variation (RDWCV); White Blood Cell Count (WBC)]

CPT Coding: 86355, 86356, 86357, 86359, 86360

Parkview Laboratory: Test Directory

Lymphocyte Subset Panel with Natural Killer Cells

PRODUCTION SCHEDULE