Last Modified:
5/11/2023 10:05:37 AM
Medical Necessity Documentation:
Client Notes:
Patient Preparation:
Specimen Requirements:
1.5 mL Frozen Plasma from a Blue Top Tube - 3.2% Na Citrate in a Plastic Vial
Collection Instructions:
- Please submit a separate, frozen vial for each special coagulation assay ordered.
- Draw blood in a light blue-top tube containing 3.2% sodium citrate
- mix gently by inverting 3-4 times.
- Centrifuge 15 minutes at 1500 g within one hour of collection.
- Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial.
- Centrifuge a second time and transfer platelet-poor plasma into a new plastic vial(s).
- Freeze immediately and transport on dry ice.
Minimum Volume:
0.5 mL Frozen Plazma
Transport & Storage: Temperature/Stability:
30 days Frozen
Rejection Criteria:
Hemolysis
Reference Range:
≤40 seconds
Critical Ranges:
Test Comments:
If PTT-LA Screen is prolonged (>40 seconds), then Hexagonal Phase Confirmation will be performed at an additional charge
Methodology:
Clot Detection
Clinical Significance:
PTT-LA with Reflex to Hexagonal Phase Confirmation - The PTT-LA is a sensitive, but non-specific assay for Lupus anticoagulants (LA). The Hexagonal Phase confirm is a specific LA test. As international guidelines recommend a multi-test approach to LA detection due to antibody heterogeneity, a comprehensive panel is available (Lupus anticoagulant Comprehensive Evaluation). Lupus anticoagulant Comprehensive Evaluation includes the PTT-LA with reflex to Hexagonal Phase Confirm, plus the DRVVT pathway of testing.
Note: Target specific anticoagulant therapy (anti-Xa medications, direct thrombin inhibitors) may cause false positive results.
Documentation:
Custom Panel:
No
PRODUCTION SCHEDULE
Turn Around Time:
1 to 4 days
Days Performed:
Monday, Tuesday, Wednesday, Thursday, Friday
Sites Performed:
Quest - Chantilly
PHL Test Code:
MSOT
EPIC Test Code:
MISC
Send Out Test Code:
17408
Alternate Test Names:
Staclot LA; Lupus Anticoagulant; Staclot-LA
Included Tests:
CPT Coding:
85730 (R 85598)
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