Activated Partial Thromboplastin Time (APTT); Basic Metabolic Panel (BMP) [Blood Urea Nitrogen (BUN); Calcium (Ca); Creatinine; Electrolytes [Carbon Dioxide (CO2); Chloride (CL); Potassium (K); Sodium (Na)]; Glucose]; Complete Blood Count with Differential [Automated Differential; Hematocrit (Hct); Hemoglobin (Hgb); Mean Corpuscular Hemoglobin (MCH); Mean Corpuscular Hemoglobin Concentration (MCHC); Mean Corpuscular Volume (MCV); Platelet (PLT); Red Blood Cell Count (RBC); Red Cell Distribution Width Standard Deviation (RDWSD); Red Cell Distribution Width Coefficient Variation (RDWCV); White Blood Cell Count (WBC)]; Prothrombin Time (PT)
Patients that are younger than 12 years will not have the alcohol tested.
* This volume does not allow for repeat testing
Specimens must be filled appropriately see this example
Correct ratio of Blood to Citrate is critical (9:1). Specimens must be filled within +/- 10% of stated volume.
If a Blue Top Tube is collected utilizing a butterfly, a Blue Discard Tube must be drawn first to remove air from the line. If this is not done, the Blue Top Tube will not be filled properly due to the vacuum in tube and a redraw will be required
Note:
Collection of blood for coagulation testing through intravenous lines that have been previously flushed with heparin should be avoided, if possible.
If the blood must be drawn through an indwelling catheter, possible heparin contamination and specimen dilution should be considered.
When obtaining specimen from indwelling lines that may contain heparin, the line should be flushed with 5mL of saline, and the first 5 mL of blood or 6-times the line volume (dead space volume of the catheter) be drawn off and discarded before the coagulation tube is filled. For those samples collected from a normal saline lock (capped off venous port), twice the dead space volume of the catheter and extension set should be discarded.
Heparinized patient specimens must be spun within 1 hour of collection - Follow Platelet Poor Collection
Clotted samples
Improperly filled tubes (under or overfilled)
Wrong anticoagulant (3.8% sodium citrate is NOT acceptable)
Patients with hematocrit (HCT) >55% (red cell volume is >1/2 tube volume)
Hemolyzed samples
Whole blood that was refrigerated or placed on ice
DO NOT freeze in a frost-free freezer
Frozen samples that were thawed in transport, or thawed samples that were refrozen
This tube must be transported to testing laboratory within 45 minutes from collection.
If this is not possible, follow these steps:
1. Heparinized patient specimens must be spun within 1 hour of collection, following these Platelet Poor Plasma preparation instructions
2. Send the platelet poor plasma and primary tube to the testing laboratory
3. Contact the laboratory at 266-1500 (Option 1) to notify PRMC laboratory that the specimen is coming
Centrifuge and separate from cells within 4 hours of collection
Hemolysis
Use Betadine or Zephiran as a skin preparation for drawing specimen
* This volume does not allow for repeat testing
Mix tube by inversion 5 times
Do not centrifuge
Specimens must not be clotted. Overfilling or underfilling may allow blood to clot. Specimens may be rejected due to improper storage or age of specimen
Clotted specimens
See Individual Tests
See Individual Tests
SUPER STAT ELIGIBLE at PRMC & PVH ONLY
01429516, 01429517
86364 x 2, 80048, 82150, 85025, 85610, 85730 (Reflex 85007 or 85008)