Lymphocyte Proliferation Panel for Mitogens and Antigens

Last Modified: 7/18/2016 8:26:43 AM


Specimen Requirements: Whole Blood in a Green Top Tube(s) - Na Heparin number of tubes based on needed volume for age of patient.

Specimen Volume:

<3 months:          3 mL
3-24 months:       5 mL
25 months-5 yrs: 6 mL
6-18 years:          8 mL
>18 years:           30 mL

Collection Instructions:

Specimen must arrive to Mayo laboratory within 24 hours of draw and by 10 a.m. on Friday. 
Draw and Send specimen Monday through Thursday only. 

No not centrifuge or aliquot

Reference Range:
LYMPHOCYTE PROLIFERATION TO ANTIGENS
Viability of lymphocytes at day 0: > or =75.0%
Maximum proliferation of Candida albicans as % CD45: > or =5.7%
Maximum proliferation of Candida albicans as % CD3: > or =3.0%
Maximum proliferation of tetanus toxoid as % CD45: > or =5.2%
Maximum proliferation of tetanus toxoid as % CD3: > or =3.3%
 
LYMPHOCYTE PROLIFERATION TO MITOGENS
Viability of lymphocytes at day 0: > or =75.0%
Maximum proliferation of phytohemagglutinin as % CD45: > or =49.9%
Maximum proliferation of phytohemagglutinin as % CD3: > or =58.5%
Maximum proliferation of pokeweed mitogen as % CD45: > or =4.5%
Maximum proliferation of pokeweed mitogen as % CD3: > or =3.5%
Maximum proliferation of pokeweed mitogen as % CD19: > or =3.9%
Methodology: Flow Cytometry
Clinical Significance:

Useful:
Evaluating patients suspected of having diminished cellular immune function
Evaluating patients with primary and secondary immunodeficiency diseases that affect T lymphocytes, including combined immunodeficiency diseases (eg, severe combined immunodeficiency, cellular immunodeficiency diseases, and some patients with humoral immunodeficiency diseases (eg, common variable immunodeficiency)
Evaluating functional T-cell recovery post-hematopoietic stem cell transplant or immunosuppressive therapy for solid-organ transplantation or in other clinical contexts

 
Custom Panel: No

PRODUCTION SCHEDULE

Turn Around Time: 14 to 21 days
Days Performed: Monday, Tuesday, Wednesday, Thursday
Sites Performed: Mayo Laboratory
PHL Test Code: MSOT
EPIC Test Code:
Send Out Test Code: 60593
Alternate Test Names: Blastogenesis Antigens; Blastogenesis Mitogens; Immune Competence; Lymphocyte Blastogenesis Antigen; Lymphocyte Blastogenesis Mitogens; Lymphocyte Function Test; Lymphocyte Phytohemagglutiin; Lymphocyte Proliferation Panel; Lymphocyte Transformation; Mitogen Studies; Mitogens; PHA; Phytohemagglutinin; Pokeweed; PWM
Included Tests: Lymphocyte Proliferation, Antigens, Lymphocyte Proliferation, Mitogens
CPT Coding: 86353 x 2

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