Vitamin C

Last Modified: 7/11/2023 9:26:01 AM


Medical Necessity Documentation:  
Client Notes:  Due to transport/storage temperature, specimen must be collected at a Parkview Hospital Lab
Patient Preparation:
Overnight Fasting is preferred.

Patient should refrain from taking Vitamin C supplements or fruits 24 hours before collection.
Specimen Requirements:

2.0 mL Frozen Serum from a Red Top Tube in an Amber Vial 

Collection Instructions: 1. Collect blood into a light protected Red Top Tube.
2. Allow to clot at ambient temperature for 20-30 minutes. 
3. Must be centrifuged at 1000g (2000-2500 rpm) and at (2-8o) for 8-10 minutes. 
4. Pour the supernatant liquid into an amber polypropylene or polethyleen tube to protect sample from light. 
5. Place dry ice or ultra-low freezer (-60° C to -90° C). to be don within 3 hours of collection

Protect from Light by placing the serum in an Amber Vial

Do not Thaw
Minimum Volume: 1.0 mL
Transport & Storage: Temperature/Stability: 28 days Frozen by dry ice or ultra-low freezer (-60° C to -90° C)

If immediate storage in (-60° C to -90° C) is not available, temporarily (up to 8hrs) transfer specimen to -20°immediately after processing and transfer onto dry ice at the earliest possible opportunity.
Rejection Criteria: Not light protected • EDTA plasma • Lithium heparin plasma • Thawed samples • Lipemic • Hemolysis • Received room temperature • Received refrigerated
Reference Range:

Male: 0.3 - 2.7 mg/dL
Female: 0.2 - 2.1 mg/dL

Critical Ranges:  
Test Comments:  
Methodology: Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS)
Clinical Significance: Vitamin C is an antioxidant involved in connective tissue metabolism, drug-metabolizing systems, and mixed-function oxidase systems to list a few. Vitamin C deficiency causes scurvy; manifestations include impaired formation of mature connective tissue, bleeding into the skin, weakness, fatigue, and depression.
Documentation:  
Custom Panel: No

PRODUCTION SCHEDULE

Turn Around Time: 5 to 8 days
Days Performed: Monday, Tuesday, Wednesday, Thursday, Friday
Sites Performed: Quest - Chantilly
PHL Test Code: VITAC
EPIC Test Code: LAB671
Send Out Test Code: 929
Alternate Test Names: Ascorbic Acid
Included Tests:  
CPT Coding: 82180

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