Medical Necessity Documentation:
Client Notes:
Patient Preparation:
Specimen Requirements:
3.0 mL nasopharyngeal aspirate/wash in VCM
Collection Instructions:
To maintain optimum viability, place swab or fluid into VCM (equal volumes of fluid and VCM)
Minimum Volume:
1.0 mL Aspirate/Wash
Transport & Storage: Temperature/Stability:
Best recovery is obtained when the specimens are refrigerated at 2-8ºC or kept on wet ice following collection and while in transit.
If there will be a long delay before processing, specimens in VCM or equivalent should be frozen at -70ºC or colder and transported on dry ice. Storage or transport at - 20ºC is not acceptable.
Rejection Criteria:
< 2 year old patients • Dry Swabs • Received in any fixative • Ambient or Frozen @-20 • Non Respiratory Specimens • In non viral transport medium such as nucleic acid or bacterial transport media
Reference Range:
Negative
Critical Ranges:
Test Comments:
If the Rapid Respiratory DFA Viral Screen is positive, the individual virus antigens (Adenovirus, Influenza A, Influenza B, Parainfluenza 1, Parainfluenza 2, Parainfluenza 3, and RSV) will be performed at an additional charge (CPT code(s): 87260, 87276, 87275, 87279 x3, 87280).
Methodology:
Direct Immunofluorescence Assay (DFA)
Clinical Significance:
Respiratory Virus Screen, DFA with Reflex to ID [14860X] - Influenza viruses are markedly epidemic and known to cause pandemics. "Influenza season" takes place during colder weather, generally regarded as October-May. Complications are a concern in the young, the elderly and in persons with chronic cardio-pulmonary diseases. Superinfections with other viruses and bacteria may occur. Parainfluenza viruses cause croup and pneumonia in children under the age of 5 and are one of the leading causes of lower respiratory illness. These cause upper respiratory illness in adults.
RSV causes mild infections in immunocompetent adults and bronchiolitis and pneumonia in infants, young children and immunocompromised/immunosuppressed adults. Simultaneous co-infections of parainfluenza 3 and RSV have been well documented.
Adenovirus may cause respiratory illness as well as hepatitis, meningitis, encephalitis, diarrhea and rash. The virus may be shed over long periods of time and may be found in respiratory, eye, fecal or urine specimens
Documentation:
Custom Panel:
No
PRODUCTION SCHEDULE
Turn Around Time:
2 -3 days
Days Performed:
Monday, Tuesday, Wednesday, Thursday, Friday
Sites Performed:
Quest - Chantilly
PHL Test Code:
RVDS
Send Out Test Code:
15020
Alternate Test Names:
Rapid Viral Respiratory DFA Screen with Reflex; Replacement for Soft Code VIRUS; Virus Culture, Comprehensive:Temporary Replacement
Included Tests:
CPT Coding:
87299; 87260; 87275; 87276; 87276; 87280