Patient Preparation
Patient should discontinue taking Biotin, Vitamin B7, or Vitamin H 48 hours before getting blood drawn
Specimen Requirements
3.0 mL Platelet Poor Plasma from a Blue Top Tube - 3.2% Na Citrate in a Plastic Vial
Transport And Storage
Unacceptable Ambient
Unacceptable Refrigerated
30 days Frozen
Collection Instructions
Draw blood in light blue-top tube containing 3.2% sodium citrate, and mix gently by inverting 3-4 times.
Centrifuge 15 minutes at 1500 x g within 1 hour of collection. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet
buffy layer and place into a plastic vial.
Centrifuge a second time and transfer platelet-poor plasma into a new plastic vial(s).
Freeze immediately and transport on dry ice
Minimum Volume
1.0 mL Platelet Poor Plasma
Neonatal Volume