Platelet Autoantibodies

Last Modified: 8/21/2023 2:31:35 PM


Medical Necessity Documentation:  
Client Notes: Contact our Client Response Department at 266-1500 (Option 1) and request a courier pickup.

Blood Center Requisition for PLT Ab testing
Patient Preparation:  
Specimen Requirements: 40.0 mL Whole Blood in five Yellow Top Tubes - ACD-A
Collection Instructions:

Collect Monday through Thursday Only - Must be sent same day as collection

Patient Platelet Count < 100,000 per mm to Volume of Whole Blood 40.0 mL
Patient Platelet Count > 100,000 per mm to Volume of Whole Blood 10.0 mL
Minimum Volume: 10.0 mL
Transport & Storage: Temperature/Stability: 4 days Refrigerated
Reference Range: Negative - ELISA values less than twice the normal control values
Positive - ELISA values greater than twice the normal control values
Critical Ranges:  
Test Comments:

Positive or negative results should be used in conjunction with clinical findings and other test results to establish diagnosis. Autoantibodies present at reduced levels, as in AITP responsive to therapy, may be missed in this assay. This assay detects only antibodies reactive with platelet GPIIb/IIIa, GPIb/IX and GPIa/IIa. Human anti-mouse antibodies may be detected, causing false positive results. 

Samples from patients recently transfused may reflect characteristics of donor, rather than patient, platelets. If the patient has been transused, wait 4 days before drawinging sample. If the patient is receiving continous platelet transusion draw sample immediately before next transfusion. Steroids or IVIg do not affect testing.
Methodology: ELISA
Clinical Significance: Autoimmune Thrombocytopenic Purpura (AITP) is one of the most common causes of immune thrombocytopenia. A diagnosis of AITP is usually reached by excluding nonimmune causes of thromcytopenia such as sepsis, fever, acute leukemia, and drug-induced thrombocytopenia. Approximately 85% of AITP patients have elevations of Platelet Associated IgG (PAIgG), PAIgM or both. The majority of these antibodies react with platelet surface membrane glycoproteins
Documentation:  
Custom Panel: No

PRODUCTION SCHEDULE

Turn Around Time: 4 to 7 days
Days Performed: Monday, Tuesday, Wednesday, Thursday, Friday
Other Testing Facility: Blood Center of Wisconsin
PHL Test Code: PLTAU
EPIC Test Code: IMO174
Send Out Test Code: 5544
Alternate Test Names: Platelet Associated IgG & IgM
Included Tests:  
CPT Coding: 86022, 86023

Go back to the top of the page.