Last Modified:
9/8/2021 10:06:25 AM
Specimen Requirements:
Nasopheryngeal specimen in a Blue Capped E-Swab® (with Liquid Amies)
Collection Instructions:
Swab tips may be polyester, rayon, nylon flocked. Flexible shafts must be used for proper collection. Accuracy of the test depends on the collection of ciliated epithelial cells material. Half the distance from the nostril to the base of the ear could be used as estimation of the site of collection. Gently insert swab into the nostril. Keep the swab near the septum floor of the nose while gently pushing into the posterior nasopharynx. Rotate swab 2-3 times and hold in place for few seconds for optimal collection. Collection of both nostrils is recommended but not required.
Nasalpharyngeal Collection Instructions
Minimum Volume:
0.3 mL NP Aspirate/Wash
Transport & Storage: Temperature/Stability:
7 days Ambient
7 days Refrigerated
30 days Frozen
Ship swab refrigerated (cold packs).
Rejection Criteria:
Collection tubes with Amies gel or charcoal containing media • Samples collected using cotton or calcium alginate tipped swabs • Rigid shaft swabs
Reference Range:
B. pertussis DNA: Not Detected
B. parapertussis DNA: Not Detected
Test Comments:
This test detects but does not distinguish between B. Pertussis and B. Holmesii.
Methodology:
Real-Time PCR
Clinical Significance:
Bordetella pertussis is the cause of whooping cough that may occur in unimmunized individuals. B. parapertussis is a related organism that causes a similar, but milder, disease. Laboratory diagnosis may require both culture and serological confirmation, although culture is difficult.
Custom Panel:
No
PRODUCTION SCHEDULE
Turn Around Time:
1 to 2 days
Days Performed:
Sunday, Monday, Tuesday, Wednesday, Thursday, Friday, Saturday
Sites Performed:
Quest - Chantilly
PHL Test Code:
MSOT
EPIC Test Code:
MISC
Send Out Test Code:
11365
Alternate Test Names:
B. pertussis/para DNA, QL Real-Time PCR; B.PERTUSSIS DNA, PCR; Bordetella pertussis/parapertussis DNA, Qual R-T P
Included Tests:
Bordetella pertussis DNA; Bordetella parapertussis DNA
CPT Coding:
87798 x 2
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