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Acid Fast Bacilli Culture

Last Modified: 3/25/2009 6:41:56 PM


Client Notes: Please contact Microbiology at 373 - 9490 if susceptibility studies are needed. Susceptibilities must be requested for organisms other than M. tuberculosis
Specimen Requirements: > 5.0 mL of  (Respiratory Secretions, Gastric, Specimens from Normally Sterile Sites) in sterile container 
or 
40.0 mL Urine in sterile container
or
Tissue/Lymph node in sterile contain
Collection Instructions:
Sputums may be collected in sputum collection device per instructions. (First AM, deep cough specimens are best). Transport at room temperature. 

Urine should be first/early AM collection

Swab specimens are not recommended.
Temperature & Stability: 2 hours Ambient
24 hours Refrigerated
4 hours Refrigerated (For Gastric Specimens)
Reference Range: Negative
Test Comments:
Specimen will be processed to isolate Mycobacteria.

Specimens are inoculated onto artificial growth media and incubated sufficient time to allow growth of the suspected microbe. Media type, incubation conditions and period of incubation may vary with the type of microorganism suspected

Smear results are reported within 24 hours of receipt of microbiology

Positive results are rendered as soon as available either as preliminary (partial) or final (complete) reports.

Preliminary negative results are rendered when sufficient incubation time has elapsed without growth on culture media capable of supporting growth of the suspected organism; and reported at 3 weeks.
Methodology: Automated and manual culture; Auramine/Rhodamine fluorescent stain

PRODUCTION SCHEDULE

Turn Around Time: 6 weeks
Days Performed: Sunday, Monday, Tuesday, Wednesday, Thursday, Friday, Saturday
Sites Performed: Parkview Central Laboratory
PHL Test Code: CXAFB
Alternate Test Names: Acid Fast Bacillus ; AFB; Culture, Acid Fast Bacilli; Mycobacteria
Included Tests: Acid Fast Bacilli Culture; Acid Fast Bacilli Stain
CPT Coding: 87015, 87116, 87206

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