Acid Fast Bacilli Culture

Last Modified: 2/4/2019 4:20:05 PM


Medical Necessity Documentation:  
Client Notes: Please contact Microbiology at 266-1500 (Option 1)if susceptibility studies are needed. Susceptibilities must be requested for organisms other than M. tuberculosis
Patient Preparation:  
Specimen Requirements: > 5.0 mL of  (Respiratory Secretions or Gastric) in sterile container 
or 
40.0 mL Urine in sterile container
or
2.0 mL CSF
or
> 50.0 mL of Fluid in a sterile container
or
Tissue/Lymph node in sterile container
Collection Instructions:
Sputums may be collected in sputum collection device per instructions. (First AM, deep cough specimens are best). 

It is recommended that 3 samples be collected 8 TO 24 HOURS apart and to include at least one early morning specimen

Urine should be first/early AM collection

Swab specimens are not recommended.

For patient collection instruction sheet on Expectorated Sputum Collection see Addendum D 
For Spanish patient collection instruction sheet on Expectorated Sputum Collection see Addendum D
For Burmese patient collection instruction sheet on Expectorated Sputum Collection see Addendum D
For Arabic patient collection instruction sheet on Expectorated Sputum Collection see Addendum D
Minimum Volume: 1.0 mL CSF
Transport & Storage: Temperature/Stability: 2 hours Ambient
24 hours Refrigerated
4 hours Refrigerated (For Gastric Specimens)
Reference Range: Negative
Critical Ranges:  
Test Comments:
Specimen will be processed to isolate Mycobacteria.

Specimens are inoculated onto artificial growth media and incubated sufficient time to allow growth of the suspected microbe. Media type, incubation conditions and period of incubation may vary with the type of microorganism suspected

Smear results are reported within 24 hours of receipt of microbiology

Positive results are rendered as soon as available either as preliminary (partial) or final (complete) reports.

Preliminary negative results are rendered when sufficient incubation time has elapsed without growth on culture media capable of supporting growth of the suspected organism; and reported at 3 weeks.
Methodology: Automated and manual culture; Auramine/Rhodamine fluorescent stain
Clinical Significance:  
Documentation:  
Custom Panel: No

PRODUCTION SCHEDULE

Turn Around Time: 6 weeks
Days Performed: Sunday, Monday, Tuesday, Wednesday, Thursday, Friday, Saturday
Sites Performed: Parkview Regional Medical Center
PHL Test Code: CXAFB
EPIC Test Code: LAB877
Alternate Test Names: Acid Fast Bacillus ; Acid Fast Culture; AFB; Culture, Acid Fast Bacilli; Mycobacteria
Included Tests: Acid Fast Bacilli Culture; Acid Fast Bacilli Stain
CPT Coding: 87015, 87116, 87206

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