Mycobacteria, Culture, with Fluorochrome Smear

Last Modified: 9/20/2022 10:46:00 AM

Medical Necessity Documentation:  
Client Notes: Please contact Microbiology at 266-1500 (Option 1) if susceptibility studies are needed. Susceptibilities must be requested for organisms other than M. tuberculosis
Patient Preparation:  
Specimen Requirements: Preferred Specimen(s):
10.0 mL of body fluids (lower respiratory tract specimens, or gastric lavage) in sterile container
2.0 mL CSF
40.0 mL urine in sterile, leak-proof container
2 grams fresh (unfixed) tissue in sterile container
1 mL exudate, aspirate, lesion material, ocular specimen collected in a sterile, leak-proof container

Minimum Volume:

2 mL of body fluids, lower respiratory tract specimens, or gastric lavage in sterile container
1 mL CSF
1 gram fresh tissue (unfixed)
20 mL urine
1 mL or 1 swab wound, exudates, aspirates, lesion material, or ocular specimens
Collection Instructions:
Tissue and biopsy material: Sterile, leak-proof container with small amount of saline, no fixative or preservative

Gastric lavage fluid: Sterile, leak-proof container.  Must be neutralized with sodium bicarbonate within 4 hours of collection.

Expectorated Sputums Instruct patient to gargle with water and cough deeply.  May be collected in sputum collection device per instructions. (First AM, deep cough specimens are best). 
Three separate early morning collections on at least 8 TO 24 HOURS apart three consecutive days are recommended.and to include at least one early morning specimen

Urine should be first/early AM collection

Swab specimens are not recommended.

For patient collection instruction sheet on Expectorated Sputum Collection see Addendum D 
For Spanish patient collection instruction sheet on Expectorated Sputum Collection see Addendum D
For Burmese patient collection instruction sheet on Expectorated Sputum Collection see Addendum D
For Arabic patient collection instruction sheet on Expectorated Sputum Collection see Addendum D
Transport & Storage: Temperature/Stability: Unacceptable Ambient
5 days Refrigerated (Transport cold packs)
Unaccetable Frozen
Rejection Criteria: 24 hour pooled urine or sputum, dry swabs, specimens received in alcohol, formalin, EDTA, lithium heparin or conventional blood culture bottles, throat/oral/sputum swabs from non-cystic fibrosis patients, urine specimens in preservative tubes
Reference Range: See Laboratory Report
Critical Ranges:
Test Comments:
Methodology: Continuous Monitoring Broth Cult System & Conventional Culture • Microscopic Exam w/ Fluorochrome St
Clinical Significance: Mycobacteria, Culture, with Fluorochrome Smear - There are more than 70 species of mycobacteria of which the primary pathogen is M. tuberculosis. Tuberculosis is a chronic, granulomatous disease caused by M. tuberculosis. Identification, and, if appropriate, antibiotic sensitivity, is required to initiate necessary therapy 
Custom Panel: No


Turn Around Time: Smear 24 hrs w/in receipt of lab, Cult 6-8 weeks
Days Performed: Sunday, Monday, Tuesday, Wednesday, Thursday, Friday, Saturday
Sites Performed: Parkview Regional Medical Center, Quest - Chantilly
PHL Test Code: CXAFB
EPIC Test Code: LAB877
Send Out Test Code: 5202
Alternate Test Names: Acid Fast Bacilli Culture; Acid Fast Bacillus ; Acid Fast Culture; AFB; Culture, Acid Fast Bacilli; Mycobacteria
Included Tests: Mycobacteria culture; Concentration; Acid-Fast Bacilli stain; 

Possible Identificaitons: M. tuberculosis Complex Probe;  M. avium Complex Probe; M. kansasii Probe; M. gordonae Probe
CPT Coding: 87015, 87116, 87206 (Identifications as performed 87149 up to x 5, I not identified 87118 or 87153) If TB isolated 1st time or after 3 months will add 87190 per drug

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