T-SPOT® TB Test

Last Modified: 10/5/2023 12:33:20 PM


Medical Necessity Documentation:  
Client Notes: The T-SPOT TB Test is only stable 32 hours, DO NOT collect before 6 a.m.
Specimen Must be received by PRMC Lab site before 4:00 pm on the date of collection. 

Contact Client Response at 266-1500 (Option 1) To acquire the needed Large 10.0 mL Green Top Tubes (Li-Hep)
or 
If an additional courier pickup will be needed
Patient Preparation:  
Specimen Requirements:

 

The T-SPOT TB Test is only stable 32 hours.  Do not collect before 6 a.m.


NOTE: Specimen Requirements may be different based on patients WBC (White Blood Cell Count). Contact Client Response at 266-1500 (Option 1) for assistance if the patients WBC count is below normal Range.

Adults & Children over 10 years old
   Whole Blood in 1 Large (10mL) FULL Green Top Tubes - Li Heparin
or   
   Whole Blood in 2 Small (6mL) FULL Green Top Tubes - Li Heparin

Children >2 to <10 years of age
   4.0 mL Whole Blood in 1 large FULL Green Top Tubes - Li Heparin 

Children under the age of <2
   2.0 mL Whole Blood in a 2 small FULL Green Top Tubes - Li Heparin

If Patients has a low WBC, please notify the laboratory with a comment on the order “low WBC, please collect 2 extra vials for Tspot testing”

 
Collection Instructions: DO NOT SPIN SAMPLE

Due to the short stability of the specimen, and the needed time to get to the reference laboratory. 

Specimens can be collected Monday thru Friday only

A patient’s cells can be pooled, if necessary to obtain sufficient cells from multiple tubes of blood which were collected and processed concurrently
Transport & Storage: Temperature/Stability: Ambient 
39 hours from collection to receipt at Quest Memphis

Specimen Must be received at PRMC Lab site before 4:00 pm on the date of collection

Unacceptable Frozen
Reference Range: Negative
Critical Ranges:  
Test Comments:  
Methodology: Patent Testing
Clinical Significance: The T-SPOT. TB test detects the immune response of T cells that have been stimulated by M tuberculosis antigens.  The test utilizes M tuberculosis-specific antigens that are absent from BCG strains and most non-tuberculosis mycobacteria.   The T-SPOT.TB test is an indirect test for M tuberculosis infection (including disease) and is intended for use in conjunction with risk assessment, radiography and other medical and diagnostic evaluations
Documentation:  
Custom Panel: No

PRODUCTION SCHEDULE

Turn Around Time: 1 to 3 days
Days Performed: Monday, Tuesday, Wednesday, Thursday, Friday
Sites Performed: Quest - Memphis
PHL Test Code: SPOT
EPIC Test Code: IMO178
Send Out Test Code: 37737
Alternate Test Names: T SPOT TB Test; TSPOT TB Test; T-SPOT TB Test
Included Tests:  
CPT Coding: 86481

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