Gliadin IgA; Gliadin IgG
Detection of antibodies to gliadin, one of the major protein components of gluten, is a sensitive assay useful in diagnosing celiac disease. However, gliadin antibodies may be found in individuals without celiac disease; thus gliadin antibody assays are less specific than assays measuring antibodies to endomysium and transglutaminase. Recent work has revealed that gliadin-reactive antibodies from celiac patients bind to a very limited number of specific epitopes on the gliadin molecule. Further, deamidation of gliadin results in enhanced binding of gliadin antibodies. Based on this information, assays using deamidated gliadin peptides bearing the celiac-specific epitopes have much higher diagnostic accuracy for celiac disease when compared to standard gliadin antibody assays.
1.0 mL Serum in a SST Gold Top Tube
48 hours Refrigerated
30 days Frozen
Specimen to be transported Refrigerated to PRMC within 48 hours of collection to be frozen by Immunology department to maintain specimen integrity until testing occurs. Do not transport Frozen.
0.5 mL Serum
Gliadin Antibody (IgG)
| <20 Units | Negative | ||
| ≥20 Units | Positive | ||
| <20 Units | Negative | ||
| ≥20 Units | Positive | ||
Detection of antibodies to gliadin, one of the major protein components of gluten, is a sensitive assay useful in diagnosing celiac disease. However, gliadin antibodies may be found in individuals without celiac disease; thus gliadin antibody assays are less specific than assays measuring antibodies to endomysium and transglutaminase. Recent work has revealed that gliadin-reactive antibodies from celiac patients bind to a very limited number of specific epitopes on the gliadin molecule. Further, deamidation of gliadin results in enhanced binding of gliadin antibodies. Based on this information, assays using deamidated gliadin peptides bearing the celiac-specific epitopes have much higher diagnostic accuracy for celiac disease when compared to standard gliadin antibody assays.
7 days
01429518; 01429519
86258 x 2