Gliadin Antibody Panel

Overview

  • EPIC Code:
  • IMO401
  • Soft Test Code:
  • GLAG
Alternate Names
  • Anti-Gliadin
  • Antigliadin Antibody
  • Gliadin Antibody IgA & IgG
Included Tests

Gliadin IgA; Gliadin IgG


Clinical Significance

Detection of antibodies to gliadin, one of the major protein components of gluten, is a sensitive assay useful in diagnosing celiac disease. However, gliadin antibodies may be found in individuals without celiac disease; thus gliadin antibody assays are less specific than assays measuring antibodies to endomysium and transglutaminase. Recent work has revealed that gliadin-reactive antibodies from celiac patients bind to a very limited number of specific epitopes on the gliadin molecule. Further, deamidation of gliadin results in enhanced binding of gliadin antibodies. Based on this information, assays using deamidated gliadin peptides bearing the celiac-specific epitopes have much higher diagnostic accuracy for celiac disease when compared to standard gliadin antibody assays.


Specimen Collection & Preparation

Specimen Requirements

1.0 mL Serum in a SST Gold Top Tube


Transport And Storage

48 hours Refrigerated
30 days Frozen

Specimen to be transported Refrigerated to PRMC within 48 hours of collection to be frozen by Immunology department to maintain specimen integrity until testing occurs. Do not transport Frozen.


Collection Instructions

 


Minimum Volume

0.5 mL Serum


Neonatal Volume

Clinical Interpretation

Reference Range:

Gliadin Antibody (IgG)

<20 Units Negative
≥20 Units Positive
Gliadin Antibody (IgA)
<20 Units Negative
≥20 Units Positive


Methodology:
  • Enzyme Immunoassay (EIA)
Clinical Significance

Detection of antibodies to gliadin, one of the major protein components of gluten, is a sensitive assay useful in diagnosing celiac disease. However, gliadin antibodies may be found in individuals without celiac disease; thus gliadin antibody assays are less specific than assays measuring antibodies to endomysium and transglutaminase. Recent work has revealed that gliadin-reactive antibodies from celiac patients bind to a very limited number of specific epitopes on the gliadin molecule. Further, deamidation of gliadin results in enhanced binding of gliadin antibodies. Based on this information, assays using deamidated gliadin peptides bearing the celiac-specific epitopes have much higher diagnostic accuracy for celiac disease when compared to standard gliadin antibody assays.


Production Schedule

Sites Performed
  • Parkview Regional Medical Center
Days Performed
Monday
Departments
  • Immunology
Turn Around Time

7 days


Coding & Compliance

CDM

01429518; 01429519


CPT Coding

86258 x 2