Herpes Simplex Virus 1 & 2 Antigen Detection

Last Modified: 10/2/2017 4:05:55 PM

Specimen Requirements: Smear/Slides
Collection Instructions:

1. Source of specimen is required, please include on requisition.
2. Two prepared slides or smears can be submitted. Fix with acetone. Place in slide holder. Ship at ambient temperature.
3. Specimens such as vesicular lesion and genital lesion can be placed into an M4 medium.
4. CSF or semen can be submitted in sterile, leak proof container. 
5. Specimens received more than 72 hours from time of draw must be frozen at -70C (not -20C) or on dry ice. Do not freeze specimen at -20 C. Virus loses infectivity.
6. M4 transport media (M4) and dacron-tipped swabs with plastic or fine-wire shafts available for use with M4 are provided

Transport & Storage: Temperature/Stability:

48 hours Ambient
72 hours Refrigerated
7 days Frozen

Rejection Criteria: Unacceptable specimens: Wooden swabs, dry swabs, and calcium alginate.
Reference Range: Not Detected
Methodology: DFA
Clinical Significance: Diagnosis of herpesvirus infection and differentiation of HSV-1 from HSV-2
Custom Panel: No


Turn Around Time: 2 to 3 days
Days Performed: Monday, Tuesday, Wednesday, Thursday, Friday, Saturday
Sites Performed: Quest - Valencia
PHL Test Code: MSOT
EPIC Test Code:
Send Out Test Code: 2426
Alternate Test Names: Herpes Simplex Virus 1 & 2 Antigen Detection [DFA]; Herpes Simplex Virus Ag by DFA
Included Tests: Herpes Simplex Virus Type 1; Herpes Simplex Virus Type 2
CPT Coding: 87273, 87274

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