Lymphocyte Panel

Overview

  • EPIC Code:
  • LAB2453
  • Soft Test Code:
  • FBCLL
Alternate Names
  • B-Cell (CLL) Panel
  • B-Cell Clonal
  • B-Cell Clonal Panel
  • B-Cell Clonal Study
  • CLL Panel
  • Flow Cytometry B-Cell Clonal
  • Lympohcyte Panel, Bone Marrow/Blood
  • Lympohcyte Panel, Flow Cytometry
Included Tests

CBC [Automated Differential; Hematocrit (Hct); Hemoglobin (Hgb); Mean Corpuscular Hemoglobin (MCH); Mean Corpuscular Hemoglobin Concentration (MCHC); Mean Corpuscular Volume (MCV); Platelet (PLT);  Red Blood Cell Count (RBC); Red Cell Distribution Width Standard Deviation (RDWSD);  Red Cell Distribution Width Coefficient Variation (RDWCV); White Blood Cell Count (WBC)]; CD2; CD3; CD4; CD5; CD7; CD8; CD10; CD14; CD16; CD19; CD20; CD22; CD23; CD33; CD34; CD38; CD45; CD56; CD57; CD79b; CD117; CD200; Surface Kappa; Surface Lambda;


Clinical Significance

Useful for the investigation of lymphocytosis of unknown origin in order to determine clonality identification of B-Cell and T-Cell chronic lymphoproliferative disorders


Specimen Collection & Preparation

Specimen Requirements

4.0 mL Whole Blood in a Green Top Tube - Na Heparin
and
3.0 mL Whole Blood in a Lavender Top Tube - EDTA


Transport And Storage

24 to 48 hours Ambient


Collection Instructions

Specimens collected with Lithium Heparin tubes will be rejected


Minimum Volume


Neonatal Volume

Clinical Interpretation

Reference Range:

Refer to Interpretive Pathology Flow Report

See CBC for its Reference Ranges


Test Comments:

The specimen is prepared by a whole blood lysis method and stained with monoclonal antibodies using a direct immunofluorescence method. Surface Kappa and Lambda light chain staining is performed with polyclonal antibodies following ammonium chloride lysis. The cells are separated by Flow Cytometry based on light scatter characteristics. The percentages of cells positive within the predominantly lymphocyte subpopulation are reported.

A Complete Blood Count will be reflexed, and charged with the ordering of this test when testing indicated


Methodology:
  • Flow Cytometry (FC)
Clinical Significance

Useful for the investigation of lymphocytosis of unknown origin in order to determine clonality identification of B-Cell and T-Cell chronic lymphoproliferative disorders


Documentation

This test was developed and the performance characteristics determined by Parkview Health Laboratories. This test has not been cleared by the U.S. Food and Drug Administration. The FDA has determined that such clearance or approval is not necessary. This test is used for clinical purposes. It should not be regarded as investigational or for research. This laboratory is certified under the Clinical Laboratory Improvement Amendments of 1988 (CLIA-88) as qualified to perform high complexity clinical laboratory testing.


Production Schedule

Sites Performed
  • Parkview Regional Medical Center
Days Performed
Monday
Tuesday
Wednesday
Thursday
Friday
Saturday
Departments
  • Flow Cytometry
Turn Around Time

48 to 72 hours


Coding & Compliance

CDM

01399703, 01399705, 01430747


CPT Coding

85025, 88184, 88185 x 23 (Reflex 85007, 85008)