Calcitrol 1,25 -Dihydroxyvitamin D

Last Modified: 10/4/2023 3:35:54 PM

Medical Necessity Documentation:  
Client Notes:  
Patient Preparation:  
Specimen Requirements:

2.0 mL Serum in a SST Gold Top Tube 

Collection Instructions: 1. Allow blood to clot (30 minutes) at ambient temperature
2. Centrifuge and separate the serum from the cells.

Avoid Hemolysis
Minimum Volume: 1.1 mL Serum
Transport & Storage: Temperature/Stability: 28 days Ambient (prefered)
28 days Refrigerated
28 days Frozen
Rejection Criteria: Grossly hemolyzed, lipemic, or icteric specimens, or plasma samples are unacceptable for this test.
Reference Range:
Vitamin D3, 1,25(OH)2
No age/sex specific ranges for this test.
Vitamin D2, 1,25(OH)2
No age/sex specific ranges for this test.
Vitamin D, 1,25(OH)2, Total, LC/MS/MS
1 year to 9 years: 31-87 pg/mL
10 years to 13 years: 30-83 pg/mL
14 years to 17 years: 19-83 pg/mL
Adults: 18-72 pg/mL 

Vitamin D3, 1,25(OH)2 indicates both endogenous
production and supplementation. Vitamin D2, 1,25(OH)2 is
an indicator of exogenous sources, such as diet or
supplementation. Interpretation and therapy are based on
measurement of Vitamin D, 1,25 (OH)2, Total.
Critical Ranges:  
Test Comments:  
Methodology: Liquid Chromatography/Tandem Mass Spectrometry
Clinical Significance:
This test measures the bioactive form of vitamin D. It is used in the differential diagnosis of hypocalcemia and to monitor patients with renal osteodystrophy or chronic renal failure. This test is not suitable for diagnosis of vitamin D deficiency and monitoring supplementation in most patients. The 25-hydroxyvitamin D test is the recommended test for those purposes (N Engl J Med. 2007;357:266-281).
25-OH Vitamin D level is performed by PHL and should be ordered as test code VD251.  1,25-dihydroxy Vitamin D is performed by Quest Diagnostics and should be ordered as test code VITD2.
Custom Panel: No


Turn Around Time: 5 to 8 days
Days Performed: Monday, Tuesday, Wednesday, Thursday, Friday, Saturday
Sites Performed: Quest - Chantilly
PHL Test Code: VITD2
EPIC Test Code: LAB536
Send Out Test Code: 16558
Alternate Test Names: 1, 25-Dihydroxyvitamin D; Calcitrol Level; Vit D1, 25 Dihydroxy; Vitamin D 1, 25 Dihydroxy ; Vitamin D, 1,25-Dihydroxy, LC/MS/MS ; Vitamin D-1, 25
Included Tests:  
CPT Coding: 82652

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