P2Y12 Platelet Reactivity

Client Notes:

Before collecting the specimen please call for OnDemand Courier @266-1500 (option1)

Patient Preparation

Manufacturer recommended Test Timing based on therapy and dose

 

Specimen Requirements

Special Collection Kit 

One (1) 6.0 mL Whole Blood in Clear Red Top Tube - No Additive, FOR LINE DRAWS ONLY,

One (1) 3 ml clear red top tube- no additive

Two (2) 2.0 mL Whole Blood Dark Blue Top Tubes - 9NC Coagulation Sodium Citrate 3.2%

Alternate Specimen

TransportAndStorage

 Kit must be received by the PRMC Lab within 3 hours of collection

Collection Instructions

Specimens to be collected with a 21g needle only.  
    (Specimens may be collected from indwelling catheter (arterial or venous) if 21G or larger bore needle is used and at least 5.0 mL whole blood is discarded PRIOR to collection of kit. Note: first tube of kit a no additive 6.0 ml tube. This means that drawing from a line would require 5.0 ml discard + all three tubes in the collection kit.)

Do Not Uncap Tubes 

Collect any additional blood tests ordered after this kit is drawn

Click here for collection instructions: 
 1. Read all instructions before collecting samples. Collect specimens using 21 g needle only. Do Not Uncap tubes. Collect addition blood tests after this kit is drawn. 
 2. Collect all tubes in number order 1-3. Blue-Top tubes are 2mL draw fill to black line (1/2 full). Do not underfill. 
 3. Gently invert tubes 5 times Do Not create bubbles. 
 4. Label tubes with patient name, DOB, date and time of collection, and collectors employee id. Return kit tubes to original baggie. Send any additional tubes collected separately. Do Not Refrigerated. 
 5. Hand deliver STAT to lab. Do Not send via pneumatic tube system (CTS). Specimen must be in PRMC Lab within 3 hours of draw.

Minimum Volume

Neonatal Volumne

Reference Range:

PRU=P2Y12 Reaction Units P2Y12 Platelet Reactivity is intended to measure the level of platelet P2Y12 receptor blockage resulting from P2Y12 receptor blocker drugs, such as clopidogrel and prasugrel. The PRU reference range in P2Y12 receptor blocker drug-naive population is 182-335, according to manufacturer's package insert. In general, PRU values lower than 180 are associated with expected antiplatelet effect. Factors that contribute to an inadequate response are drug interactions, underlying health conditions, non-compliance, and inherent platelet disorders. Drug response is also dependent on dosage and timing of testing. Patients taking P2Y12 inhibitors are at increased risk of perioperative bleeding due to platelet dysfunction from drug effect. It is recommended to discontinue use of P2Y12 drugs for 5-7 days prior to surgery to allow restoration of platelet function. PRU values greater than or equal to 180 are indication of no drug effect. This assay does not test aspirin drug response or platelet dysfunction unrelated to P2Y12 receptor blockage. Clinical decisions cannot be based solely upon this test result.

Critical Ranges:

No critical limits have been established for PRU test result

Methodology:

• Whole Blood aggregometry (platelet inhibition) VerifyNow P2Y12 assay

CDM

01425576

CPT Coding

85576