Alpha-Fetoprotein Maternal, 2.5 MoM

Last Modified: 4/10/2018 8:02:21 AM


Specimen Requirements:

3.0 mL Serum in a Red Top Tube

Collection Instructions: Collect specimen between 14-22 weeks gestation

Split serum into two tubes

Alpha-Fetoprotein 2-part Request Form must accompany the specimen (See Addendum A - AFP Form Example)
Minimum Volume: 1.5 mL Serum
Transport & Storage: Temperature/Stability: 14 days Ambient
14 days Refrigerated
45 days Frozen

Ship on cold packs. If not shipped immediately, samples should be frozen.
Rejection Criteria: Icteric or grossly hemolyzed specimens
Reference Range: Refer to Interpretive Results
Test Comments:

Results which are less than 2.5 multiples of the median (MoM) are considered to be normal when evaluating fetuses for neural tube defects (NTD). Normal serum AFP values exclude 95% of cases of anencephaly and around 80% of cases of spina bifida. Closed dysraphic states and small defects may yield normal results. Unexplained elevations of MSAFP have been associated with adverse pregnancy outcomes such as premature rupture of the membranes, fetal demise, abruptio placenta, pregnancy - induced hypertension, preterm labor and neonatal death. However, the effectiveness of monitoring or intervention based upon such information is not known.
Maternal serum AFP tests are not diagnostic and AFP alone has limited application for screening purposes. This panel should only be used to screen for Neural Tube Defects. AFP Quad Screen (test code #3092-PHL Code QUAD) is preferred when screening for fetal anomalies.

Methodology: Immunoassay (IA)
Clinical Significance: Maternal serum alpha-fetoprotein (MSAFP) is used for prenatal screening of open neural tube defects (ONTD). MSAFP screening detects 88% of anencephaly and 79% of open spina bifida with a 3% false positive rate. Normal results do not ensure birth of a normal infant. In addition, 2-3% of newborns have some type of physical or mental defect, many of which may be undetectable with current prenatal diagnostic procedures.

Limitations

MSAFP results consistent with increased risk of NTD may be followed-up with appropriate diagnostic testing as detailed in the report. MSAFP does not screen for chromosomal abnormalities.

Documentation: AFP Quad Screen is preferred when screening for fetal anomalies.
Custom Panel: No

PRODUCTION SCHEDULE

Turn Around Time: 2 to 3 days
Days Performed: Sunday, Monday, Tuesday, Wednesday, Thursday, Friday, Saturday
Sites Performed: Quest - Valencia
PHL Test Code: MSOT
EPIC Test Code:
Send Out Test Code: 3090
Alternate Test Names: AFP; Maternal Serum AFP
Included Tests: AFP, Maternal Risk Interpretation
CPT Coding: 82105

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