Haemophilus influenzae B Antibody IgG

Clinical Significance

Specimen Requirements

(2) 1.0 mL Serum from a Red Top Tube in a Plastic Vial
    1 Pre Specimen
    1 30 day Post Specimen

Transport And Storage

7 days Ambient
14 days Refrigerated
30 days Frozen

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles) 

Collection Instructions

Serum separator tube. "Pre" and 30-day "post" Haemophilus influenzae b vaccination specimens should be submitted together for testing. 
"Post" specimen should be drawn 30 days after immunization and must be received within 60 days of "pre" specimen. 

Centrifuge and separate within 2 hours of collection. 

Clearly Mark specimens clearly as "Pre-Vaccine" or "Post-Vaccine". 

Minimum Volume

0.2 mL Serum

Neonatal Volume

Reference Range:

≥1.00 mcg/mL
Interpretive Criteria

<0.15 mcg/mL	Nonprotective antibody level
0.15-0.99 mcg/mL	Indeterminate for protective antibody
≥1.00 mcg/mL	Protective antibody level

Methodology:

• Immunoassay (IA)

Clinical Significance

Documentation

Responder status is determined according to the ratio of post-vaccination concentration to pre-vaccination concentration of Haemophilus influenza b antibody, IgG as follows:
1. If the post-vaccination concentration is less than 3.0 µg/mL, the patient is considered to be a non-responder.
2. If the post-vaccination concentration is greater than or equal to 3.0 µg/mL, a patient with a ratio of greater than or equal to 4 is a good responder, a ratio of 2-4 is a weak responder, and a ratio of less than 2 is considered a nonresponder.

CDM

00913333

CPT Coding

86684