Haemophilus influenzae B Antibody IgG

Last Modified: 11/12/2019 2:56:01 PM

Medical Necessity Documentation:  
Client Notes:  
Patient Preparation:  
Specimen Requirements: (2) 1.0 mL Serum from a Red Top Tube in a Plastic Vial
    1 Pre Specimen
    1 30 day Post Specimen
Collection Instructions: Serum separator tube. "Pre" and 30-day "post" Haemophilus influenzae b vaccination specimens should be submitted together for testing. 
"Post" specimen should be drawn 30 days after immunization and must be received within 60 days of "pre" specimen. 

Centrifuge and separate within 2 hours of collection. 

Clearly Mark specimens clearly as "Pre-Vaccine" or "Post-Vaccine". 
Minimum Volume: 0.2 mL Serum
Transport & Storage: Temperature/Stability: 7 days Ambient
14 days Refrigerated
30 days Frozen

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles) 
Rejection Criteria: Gross hemolysis • Gross lipemia • Grossly icteric
Reference Range: ≥1.00 mcg/mL
Interpretive Criteria
<0.15 mcg/mL Nonprotective antibody level
0.15-0.99 mcg/mL Indeterminate for protective antibody
≥1.00 mcg/mL Protective antibody level

Critical Ranges:  
Test Comments:  
Methodology: Immunoassay (IA)
Clinical Significance:
Haemophilus influenzae is a gram-negative bacteria that is present in approximately three-quarters of children and adults. In infants and young children, haemophilus influenzae, especially type B, may cause bacteremias and meningitis. In children and older individuals haemophilus influenzae may cause respiratory tract infections.
Documentation: Responder status is determined according to the ratio of post-vaccination concentration to pre-vaccination concentration of Haemophilus influenza b antibody, IgG as follows:
1. If the post-vaccination concentration is less than 3.0 µg/mL, the patient is considered to be a non-responder.
2. If the post-vaccination concentration is greater than or equal to 3.0 µg/mL, a patient with a ratio of greater than or equal to 4 is a good responder, a ratio of 2-4 is a weak responder, and a ratio of less than 2 is considered a nonresponder.
Custom Panel: No


Turn Around Time: 1 to 4 days
Days Performed: Tuesday, Friday
Sites Performed: Quest - Chantilly
PHL Test Code: HITB
EPIC Test Code: IMO5108
Send Out Test Code: 35135
Alternate Test Names: H. influenzae Antibody; Haemophilus influenza Type b Antibody (IgG); Haemophilus influenzae B IgG Ab; Haemophilus influenzae b Vaccine Response; HIB (Haemophilus influenzae Type B) Vaccine Respon
Included Tests:  
CPT Coding: 86684

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