Haemophilus influenzae B Antibody IgG

Last Modified: 9/6/2018 3:08:25 PM


Specimen Requirements: (2) 1.0 mL Serum from a Red Top Tube in a Plastic Vial
    1 Pre Specimen
    1 30 day Post Specimen
Collection Instructions: Serum separator tube. "Pre" and 30-day "post" Haemophilus influenzae b vaccination specimens should be submitted together for testing. 
"Post" specimen should be drawn 30 days after immunization and must be received within 60 days of "pre" specimen. 

Centrifuge and separate within 2 hours of collection. 

Clearly Mark specimens clearly as "Pre-Vaccine" or "Post-Vaccine". 
Minimum Volume: 0.2 mL Serum
Transport & Storage: Temperature/Stability: 48 hours Ambient
2 weeks Refrigerated
1 year Frozen

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles) 
Rejection Criteria: Plasma or other body fluids. Contaminated, hemolyzed or, severely lipemic specimens
Reference Range: < 1.0 µg/mL = Antibody concentration not protective.
≥ 1.0 µg/mL = Antibody to H. influenzae b detected. Suggestive of protection.

Methodology: Immunoassay (IA)
Clinical Significance:
Evaluate the ability of a patient to produce antibody to a protein conjugated bacterial (H. influenza) vaccine to rule out antibody deficiency.
Documentation: Responder status is determined according to the ratio of post-vaccination concentration to pre-vaccination concentration of Haemophilus influenza b antibody, IgG as follows:
1. If the post-vaccination concentration is less than 3.0 µg/mL, the patient is considered to be a non-responder.
2. If the post-vaccination concentration is greater than or equal to 3.0 µg/mL, a patient with a ratio of greater than or equal to 4 is a good responder, a ratio of 2-4 is a weak responder, and a ratio of less than 2 is considered a nonresponder.
Custom Panel: No

PRODUCTION SCHEDULE

Turn Around Time: 1 to 4 days
Days Performed: Tuesday, Friday
Sites Performed: Quest - Valencia
PHL Test Code: MSOT
EPIC Test Code:
Send Out Test Code: 35135
Alternate Test Names: H. influenzae Antibody; Haemophilus influenza Type b Antibody (IgG); Haemophilus influenzae B IgG Ab; Haemophilus influenzae b Vaccine Response; HIB (Haemophilus influenzae Type B) Vaccine Respon
CPT Coding: 86684

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