Catecholamines Fractionation

Last Modified: 2/13/2019 3:10:10 PM


Medical Necessity Documentation:  
Client Notes:  
Patient Preparation:
Overnight Fasting preferred

It is preferable for the patient to be off medications for 3 days before specimen collection. 
However, common antihypertensives (diuretics, ACE inhibitors, calcium channel blockers, alpha and beta blockers) cause minimal or no interference. 

Patient should avoid alcohol, coffee, tea, tobacco, and strenuous exercise before collection.
Specimen Requirements:

4.0 mL Plasma from a Green Top Tube - Na Heparin in a Plastic Vial

Collection Instructions:
  1. Pre-Chill tube before collection
  2. Patient should be relaxed in either a supine or upright position before blood is drawn. (States of anxiety and stress can cause fluctuations in the catecholamine levels)
  3. Order must include body position as supine or upright when drawing patient
  4. Centrifuge for 20 minutes 1000XG.
  5. The plasma "MUST" be free of RBCS,
  6. Transfer plasma to clean, plastic, screw-capped vial(s).
  7. Freeze specimen on dry ice to transport
Minimum Volume: 2.5 mL
Transport & Storage: Temperature/Stability: 1 month Frozen
Reference Range: Epinephrine
3 years to 15 years, Supine: Less than or equal to 464 pg/mL 
Adult Supine: Less than 50 pg/mL

3 years to 15 years, Upright: Not available 
Adult Upright: Less than 95 pg/mL 

Norepinephrine
3 years to 15 years, Supine: Less than or equal to 1251 pg/mL 
Adult Supine: 112- 658 pg/mL

3 years to 15 years, Upright: Not available 
Adult Upright: 217-1109 pg/mL

Dopamine
3 years to 15 years, Supine: Less than or equal to 60 pg/mL 
Adult Supine: Less than 30 pg/mL

3 years to 15 years, Upright: Not available 
Adult Upright: Less than 30 pg/mL 

Calculated Total (E+NE) 
Adult Supine: 123- 671 pg/mL 
Adult Upright: 242-1125 pg/mL

Detection limits
Epinephrine 20 pg/mL
Norepinephrine 20 pg/mL
Dopamine 30 pg/mL
Critical Ranges:  
Test Comments: Due to stress, plasma Catecholamine levels are generally unreliable in infants and small children. Urinary Catecholamine assays are more reliable.
Methodology: High Performance Liquid Chromatography
Clinical Significance:  
Documentation: Pediatric data from J Chromatogr 1993; 617:304-307
Custom Panel: No

PRODUCTION SCHEDULE

Turn Around Time: 3 to 5 days
Days Performed: Monday, Wednesday, Friday
Sites Performed: Quest - Chantilly
PHL Test Code: CATFP
EPIC Test Code: LAB870
Send Out Test Code: 14566
Included Tests:  
CPT Coding: 82384

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