Galactose-1-Phosphate, Erythrocytes

Last Modified: 6/20/2019 8:47:52 AM


Specimen Requirements: Red Cell Pellet from an Whole Blood in a Green Top Tube - Na Heparin in a Plastic Vial
Collection Instructions:

Erthrocytes must be washed within 4 hours of draw. 

Collection:
1.) Collect and process a minimum of 2 mL of whole blood as follows: 
      A. Immediately centrifuge for 10 minutes at 1900 RPM
      B. Discard the plasma and buffy coat layers.
      C. Add a cold 0.9% saline solution to the erythrocytes (about 2 times the volume of erythrocytes). 
      D. Mix gently by inversion and centrifuge again for 10 minutes at 650 x g. 
      E. Remove and discard the saline. 
      F. Repeat the wash steps (C-E) 2 more times.
2.) After the final centrifugation, remove and discard the saline and a thin layer of the top cells.

Additional information: The relative centrifugal force (G-force) can be estimated by applying the following formula: g=11.18 x r (n/1000)2. Where: r=radius in centimeters and n=speed in RPM. The radius from the center of the rotation axis to the bottom or outermost portion of the test tube should be used. RCF is expressed relative to the force of the earth's gravity.

 
Transport & Storage: Temperature/Stability: Washed RBC - Frozen 9 days

Ambient and Refrigerated unacepptable.
Rejection Criteria: Anticoagulant other than heparin
Reference Range:
<1.0 mg/dL (non-galactosemic)
1.0-4.0 mg/dL (galactosemic on galactose restricted diet)
>4.0 mg/dL (galactosemic on unrestricted diet)
Methodology: Ultraviolet • Enzymatic
Clinical Significance:

Monitoring dietary therapy of patients with galactosemia due to deficiency of galactose-1-phosphate uridyltransferase or uridine diphosphate galactose-4-epimerase

Custom Panel: No

PRODUCTION SCHEDULE

Turn Around Time: 10 to 13 days
Days Performed: Wednesday
Sites Performed: Mayo Laboratory
PHL Test Code: MSOT
EPIC Test Code:
Send Out Test Code: 5282 / GAL1P
Alternate Test Names: Gal-1-P; Galactokinase; Galactose-1-Phosphate; Galactose-1-Phosphate, RBC; Galactosemia
CPT Coding: 84378

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