HIV-1 Genotype

Overview

  • EPIC Code:
  • IMO1958
  • Soft Test Code:
  • HIVG
  • Send Out Test Code:
  • 34949
Alternate Names
  • HIV Genotype
  • HIV Genotype Plus
  • Reverse Trans and Prot Inhibitor
Clinical Significance

Genotyping assays (HIV-1 genotype to RTI and to PRI) detect mutations that are present in the HIV reverse transcriptase and protease. This assay may not detect minor viral species that constitute less than 25% of the circulating virus population. Consultation with an expert in HIV drug resistance is encouraged to facilitate interpretation of genotype test results, and to evaluate which mutations and/or combinations of mutations are associated with drug resistance.


Specimen Collection & Preparation

Specimen Requirements

3.0 mL FROZEN Plasma from 2 Lavender Top Tubes - EDTA in a Plastic Vial


Transport And Storage

24 hours Ambient
6 days Refrigerator
42 days Frozen (Preferred)


Avoid repeated freezing and thawing of specimen. Ship frozen only.


Collection Instructions

Separate plasma from the cells by centrifugation within 24 hours after collection. 


Minimum Volume

1.0 mL Plasma


Neonatal Volume

Clinical Interpretation

Reference Range:

Refer to Interpretive Results


Methodology:
  • DNA Sequencing
  • Reverse Transcriptase Polymerase Chain Reaction (RT-PCR)
Clinical Significance

Genotyping assays (HIV-1 genotype to RTI and to PRI) detect mutations that are present in the HIV reverse transcriptase and protease. This assay may not detect minor viral species that constitute less than 25% of the circulating virus population. Consultation with an expert in HIV drug resistance is encouraged to facilitate interpretation of genotype test results, and to evaluate which mutations and/or combinations of mutations are associated with drug resistance.


Documentation

An infection is often composed of a variety of strains of the virus. The test may identify only the predominant type of virus thus leaving the minority viruses to thrive once the predominant type is treated. Lack of resistance may not confer susceptibilities.


Production Schedule

Departments
  • Sendouts - Clinical

Coding & Compliance

CDM

00912602, 00912600


CPT Coding

87900, 87901