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HIV-1 Genotype
Last Modified:
3/27/2019 3:45:40 PM
Medical Necessity Documentation:
Client Notes:
Patient Preparation:
Specimen Requirements:
3.0 mL FROZEN Plasma from a
Lavender Top Tube
- EDTA in a Plastic Vial
Collection Instructions:
Separate plasma from the cells by centrifugation within 24 hours after collection.
Minimum Volume:
1.0 mL Plasma
Transport & Storage: Temperature/Stability:
24 hours Ambient
6 days Refrigerator
42 days Frozen (Preferred)
Avoid repeated freezing and thawing of specimen.
Ship frozen only.
Rejection Criteria:
Whole Blood greater than 24 hours; frozen whole blood; serum
Reference Range:
Refer to Interpretive Results
Critical Ranges:
Test Comments:
Methodology:
DNA Sequencing • Reverse Transcriptase Polymerase Chain Reaction (RT-PCR)
Clinical Significance:
Genotyping assays (HIV-1 genotype to RTI and to PRI) detect mutations that are present in the HIV reverse transcriptase and protease. This assay may not detect minor viral species that constitute less than 25% of the circulating virus population. Consultation with an expert in HIV drug resistance is encouraged to facilitate interpretation of genotype test results, and to evaluate which mutations and/or combinations of mutations are associated with drug resistance.
Documentation:
An infection is often composed of a variety of strains of the virus. The test may identify only the predominant type of virus thus leaving the minority viruses to thrive once the predominant type is treated. Lack of resistance may not confer susceptibilities.
Custom Panel:
No
PRODUCTION SCHEDULE
PHL Test Code:
HIVG
EPIC Test Code:
IMO1958
Send Out Test Code:
34949
Alternate Test Names:
HIV Genotype; HIV Genotype Plus; Reverse Trans and Prot Inhibitor
Included Tests:
CPT Coding:
87900, 87901
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