Centrifuge light blue-top tube for 15 minutes at approximately 1500 x g within 60 minutes of collection. Using a plastic pipette, remove plasma, taking care to avoid WBS/platelet buffy layer and place
into a plastic vial. Centrifuge a second time and transfer platelet-poor plasma into a new plastic vial.
Plasma must be free of platelets (< 10,000/uL). Freeze immediately and ship on dry ice.
Avoid Hemolysis