Herpes Simplex Virus 1 & 2

Last Modified: 3/27/2019 3:38:59 PM


Medical Necessity Documentation:  
Client Notes:  
Patient Preparation:  
Specimen Requirements:  cutaneous (skin, penis, labia) or mucocutaneous (oral, ocular, urethral, nasal, vaginal cervical, rectal) lesions in M4RT Vial(Red Cap Vial)
Collection Instructions:

Specimens must be collected from cutaneous (skin, penis, labia) or mucocutaneous (oral, ocular, urethral, nasal, vaginal cervical, rectal) lesions from symptomatic patients and immediately placed in Remel Viral Transport Media--Room Temp (M4RT-red cap) .
Collection site and/or source must be given on order

Endocervical: 
1. Remove excess mucus from cervical os and surrounding  mucosa with large tipped swab.
2. Insert specimen collection swab into endocervical canal.
3. Gently rotate swab for 1Q-30 seconds to ensure adequate sampling.
4. Withdraw swab carefully to avoid vaginal mucosa.
5. Remove cap from transport vial and place swab into vial.
6. Snap off swab shaft, re-cap transport vial.

Urethral:
1. The patient should not have urinated for at least 1 hour prior to collection.
2. Insert collection swab 2 to 4 em into urethra.
3. Gently rotate swab for 2-3seconds to ensure adequate sampling.
4. Withdraw swab carefully.
5. Remove cap from transport vial and place swab into vial.
6. Snap off swab shaft, re-cap transport vial.

Transport & Storage: Temperature/Stability: 5 days Refrigerated (Transport)
30 days Frozen (-70°C)
Rejection Criteria: CSF, tissue, screening specimens in absence of lesions/symptoms; Cepheid Xpert Nasopharyngeal Swab in UTM (these are only approved for respiratory viruses), no Cepheid CT/NG transport media
Reference Range:

No HSV detected

Critical Ranges:  
Test Comments:  
Methodology: Helicase-Dependant DNA Amplification
Clinical Significance:  
Documentation:  
Custom Panel: No

PRODUCTION SCHEDULE

Turn Around Time: 1 to 3 days
Days Performed: Monday, Tuesday, Wednesday, Thursday, Friday
Sites Performed: Parkview Regional Medical Center
PHL Test Code: HSDNA
EPIC Test Code: LAB2946
Alternate Test Names: Herpes Simplex 1+2 by DNA Amplification; Herpes Simplex, HSV 1+2, Molecular Herpes Assay; HSV-1 & HSV-2
Included Tests: Herpes Simplex Virus 1 (HSV-1); Herpes Simplex Virus 2 (HSV-2);
CPT Coding: 87529

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