HPV mRNA E6/E7

Overview

  • EPIC Code:
  • LAB2956
  • Soft Test Code:
  • HPV67
  • Send Out Test Code:
  • 90887
Alternate Names
  • E6/E7
  • Hologic
  • TMA
Clinical Significance

The presence of E6/E7 messenger RNA from 14 high risk HPV types indicates incorporation of HPV DNA into the host cells. Proteins expressed from E6-E7 polycistronic mRNA alter cellular p53 and retinoblastoma protein functions, leading to disruption of cell-cycle check points and cell genome instability. This information, together with the physician’s assessment of cytology history, other risk factors, and professional guidelines, may be used to guide patient management.


Specimen Collection & Preparation

Specimen Requirements

ThinPrep® vial with a minimum of 1.5 mL Preservcyt® fluid remaining after cytology is performed, or transfer 1 mL of Preservcyt® fluid into APTIMA® specimen transfer tube (green label)


Transport And Storage

30 days Ambient (transport)
90 days Refrigerated


Collection Instructions

Please note: For clients performing their own cytology (pap testing), who choose to send HPV mRNA testing to Quest Diagnostics, the preferred specimen requirement steps should be adhered to.

1.) Collect cervical specimens in ThinPrep® Pap Test vials containing PreservCyt® Solution with broom-type or cytobrush/spatula collection devices according to the manufacturer's instructions.

2.) Alternatively, 1 mL PreserCyt® can be aliquoted pre-cytology or post-cytology..


Minimum Volume


Neonatal Volume

Clinical Interpretation

Reference Range:

Not Detected


Test Comments:

This test was performed using the APTIMA® HPV Assay (Gen-Probe Inc.).


Methodology:
  • Transcription-Mediated Amplification (TMA)
Clinical Significance

The presence of E6/E7 messenger RNA from 14 high risk HPV types indicates incorporation of HPV DNA into the host cells. Proteins expressed from E6-E7 polycistronic mRNA alter cellular p53 and retinoblastoma protein functions, leading to disruption of cell-cycle check points and cell genome instability. This information, together with the physician’s assessment of cytology history, other risk factors, and professional guidelines, may be used to guide patient management.


Production Schedule

Sites Performed
  • Quest - Chantilly
Days Performed
Tuesday
Thursday
Departments
  • Sendouts - Clinical
Turn Around Time

3 to 6 days


Coding & Compliance

CPT Coding

87624