Oxidative Burst

Last Modified: 5/20/2019 1:00:55 PM


Medical Necessity Documentation:  
Client Notes:  
Patient Preparation:  
Specimen Requirements: Two (2) 7.0 mL Whole Blood in a Green Top Tube - Na Heparin
Collection Instructions: Collect Monday through Thursday ONLY

1. Obtain peripheral blood in a sodium heparin anticoagulated tube (green-top) and send 7 mL of heparinized whole blood.
2. Obtain peripheral blood from a normal, unrelated person at the same time, in a sodium heparin tube (green-top) and send 7 mL of heparinized whole blood. Label clearly on outermost label Transport Control.
3. Draw date and time must be provided with each whole blood specimen. 4. Forward promptly at room temperature only.
Minimum Volume: Two (2) 4.0 mL Whole Blood
Transport & Storage: Temperature/Stability: 72 hours Ambient
Rejection Criteria: Hemolysis • Lipemia • Icterus • Clotted specimens • Received refrigerated • Frozen specimens
Reference Range:
%Oxidation Pos Neutrophils
24 Hours ≥93%
48 Hours ≥83%
72 Hours ≥82%
Critical Ranges:  
Test Comments:  
Methodology: Flow Cytometry
Clinical Significance: This assay measures the capability of neutrophils (PMN) to undergo oxidative metabolism to produce superoxide anion and hydrogen peroxide, as detected by oxidation of dihydrorhodamine (DHR). Patients with chronic granulomatous disease (CGD) are unable to oxidize DHR due to defects in oxidative capacity slowly decreases as the specimen age (hours post collection) increases. It is thus important that the reference range value appropriate for the specimen age be utilized when assessing the reported results.
Documentation:  
Custom Panel: No

PRODUCTION SCHEDULE

Turn Around Time: 3 to 7 days
Days Performed: Sunday, Monday, Tuesday, Wednesday, Thursday, Friday, Saturday
Other Testing Facility: Quest San Jaun Capistrano
PHL Test Code: OXIB
EPIC Test Code: LAB2720
Send Out Test Code: 37523
Alternate Test Names: Chronic Granulation; NBT (CGD-IT); Neutrophil Burst; Neutrophil Function; Neutrophil Function, Oxidative Burst ; Nitro Blue; Oxidative Burst, Neutro; Polymorphonuclear Dihydrorhodamine 123 oxidation ; Respiratory Burst Assay
Included Tests:  
CPT Coding: 82657 x 2

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