This assay measures the capability of neutrophils (PMN) to undergo oxidative metabolism to produce superoxide anion and hydrogen peroxide, as detected by oxidation of dihydrorhodamine (DHR). Patients with chronic granulomatous disease (CGD) are unable to oxidize DHR due to defects in oxidative capacity slowly decreases as the specimen age (hours post collection) increases. It is thus important that the reference range value appropriate for the specimen age be utilized when assessing the reported results.
Two (2) 7.0 mL Whole Blood in a Green Top Tube - Na Heparin
72 hours Ambient
Collect Monday through Thursday ONLY
1. Obtain peripheral blood in a sodium heparin anticoagulated tube (green-top) and send 7 mL of heparinized whole blood.
2. Obtain peripheral blood from a normal, unrelated person at the same time, in a sodium heparin tube (green-top) and send 7 mL of heparinized whole blood. Label clearly on outermost label Transport Control.
3. Draw date and time must be provided with each whole blood specimen. 4. Forward promptly at room temperature only.
Two (2) 4.0 mL Whole Blood
| 24 Hours | ≥93% |
| 48 Hours | ≥83% |
| 72 Hours | ≥82% |
This assay measures the capability of neutrophils (PMN) to undergo oxidative metabolism to produce superoxide anion and hydrogen peroxide, as detected by oxidation of dihydrorhodamine (DHR). Patients with chronic granulomatous disease (CGD) are unable to oxidize DHR due to defects in oxidative capacity slowly decreases as the specimen age (hours post collection) increases. It is thus important that the reference range value appropriate for the specimen age be utilized when assessing the reported results.
3 to 7 days
00910633
82657 x 2