Decreased fibrinogen levels are associated with increased bleeding tendencies. Increased fibrinogen levels are a risk factor for thrombotic complications.
It is recommended that an additional blue top tube be drawn for FIBR when other Coagulation testing is ordered
Whole blood is the prefered specimen. When testing will not be performed within 24 hours the whole blood must be spun to aquire a Platelet Poor Plasma specimen before submitting to testing laboratory.
* This volume does not allow for repeat testing
Specimens must be filled appropriately see this example
Correct ratio of Blood to Citrate is critical (9:1). Specimens must be filled within +/- 10% of stated volume.
If a Blue Top Tube is collected utilizing a butterfly, a Blue Discard Tube must be drawn first to remove air from the line. If this is not done, the Blue Top Tube will not be filled properly due to the vacuum in tube and a redraw will be required
Collection of blood for coagulation testing through intravenous lines that have been previously flushed with heparin should be avoided, if possible.
If the blood must be drawn through an indwelling catheter, possible heparin contamination and specimen dilution should be considered.
When obtaining specimen from indwelling lines that may contain heparin, the line should be flushed with 5mL of saline, and the first 5 mL of blood or 6-times the line volume (dead space volume of the catheter) be drawn off and discarded before the coagulation tube is filled. For those samples collected from a normal saline lock (capped off venous port) twice the dead space volume of the catheter and extension set should be discarded.
> 24 Hours from collection
HCT >55%
cell volume > 1/2 the tube volume
Hemolyzed
Clotted
Underfilled
Inappropriate temperature
* This volume does not allow for repeat testing
Specimens must be filled appropriately see this example
Correct ratio of Blood to Citrate is critical (9:1). Specimens must be filled within +/- 10% of stated volume.
If a Blue Top Tube is collected utilizing a butterfly, a Blue Discard Tube must be drawn first to remove air from the line. If this is not done, the Blue Top Tube will not be filled properly due to the vacuum in tube and a redraw will be required
Collection of blood for coagulation testing through intravenous lines that have been previously flushed with heparin should be avoided, if possible.
If the blood must be drawn through an indwelling catheter, possible heparin contamination and specimen dilution should be considered.
When obtaining specimen from indwelling lines that may contain heparin, the line should be flushed with 5mL of saline, and the first 5 mL of blood or 6-times the line volume (dead space volume of the catheter) be drawn off and discarded before the coagulation tube is filled. For those samples collected from a normal saline lock (capped off venous port) twice the dead space volume of the catheter and extension set should be discarded.
Follow Platelet Poor Processing
Freeze/thaw cycles occurred due to utilization of frost free freezer
Thawed specimen
Inappropriate temperature
Hemolyzed
Normal: 150 - 440 mg/dl
Decreased fibrinogen levels are associated with increased bleeding tendencies. Increased fibrinogen levels are a risk factor for thrombotic complications.
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