Lupus Panel 4

Overview

  • EPIC Code:
  • Soft Test Code:
  • MSOT
  • Send Out Test Code:
  • 36378
Alternate Names
  • ANAlyzer®
  • ANAlyzeR™ ANA, IFA with Reflex Titer/Pattern, Syst
  • ANAlyzeR™ ANA, IFA with Reflex Titer/Pattern, Systemic Autoimmune Panel 1
  • Antinuclear Antibody Screen,Systemic Lupus Erythematosus (SLE)
  • FANA
  • Fluorescent ANA
  • Lupus (12) Panel
  • Lupus Analyzer
  • Lupus Analyzer; Lupus Panel 4 with ANA, IFA with Reflex to Titer and Pattern
  • Lupus Panel 4 with ANA, IFA with Reflex to Titer and Pattern
  • Progressive ANA, Hep-2
  • Thyroiditis
Included Tests

ANA Screen, IFA, with Reflex to Titer and Pattern; DNA (ds) Antibody; Complement Component C3c; Complement Component C3c and C4c; Complement Component C4c; Rheumatoid Factor; Scleroderma Antibody (Scl-70); Thyroid Peroxidase Antibodies (TPO); Ribosomal P Antibody; Sm Antibody; Sm/RNP Antibody; Sjogren's Antibody (SS-A); Sjogren's Antibody (SS-B)


Clinical Significance

This panel can be helpful in the diagnosis of systemic lupus erythematosus (SLE) and several other autoimmune diseases, including mixed connective tissue disease, Sjogren syndrome, systemic sclerosis, and autoimmune thyroid disease. The panel simultaneously tests for antinuclear antibodies (ANAs) with an immunofluorescence assay (IFA), performed on human epithelial type 2 (HEp-2) cells, and 11 biomarkers associated with these conditions. For specimens with positive ANA IFA results, reflex testing for ANA titer and pattern is performed.

The ANA testing has high sensitivity (97%) for SLE but limited specificity (34%) [1]. Thus, a positive ANA test does not exclude other autoimmune diseases with similar clinical features [2]. Testing for specific antibodies in a panel may expedite the evaluation of SLE but is generally not recommended [3].

The results of this test should be interpreted in the context of pertinent clinical and family history and physical examination findings.


Specimen Collection & Preparation

Patient Preparation

Overnight fasting is preferred


Specimen Requirements

9.0 mL Serum from a Red Top Tube(s) in a Plastic Vial


Alternate Specimen


TransportAndStorage

7 days Refrigerated (transport)
30 days Frozen


Collection Instructions


Minimum Volume

6.0 mL Serum


Neonatal Volumne

Clinical Interpretation

Reference Range:

Refer to Interpretive Results


Test Comments:

  • If ANA Screen, IFA is positive, then ANA Titer and Pattern will be performed


Methodology:
  • Multiple - Based on Individual Components

Clinical Significance

This panel can be helpful in the diagnosis of systemic lupus erythematosus (SLE) and several other autoimmune diseases, including mixed connective tissue disease, Sjogren syndrome, systemic sclerosis, and autoimmune thyroid disease. The panel simultaneously tests for antinuclear antibodies (ANAs) with an immunofluorescence assay (IFA), performed on human epithelial type 2 (HEp-2) cells, and 11 biomarkers associated with these conditions. For specimens with positive ANA IFA results, reflex testing for ANA titer and pattern is performed.

The ANA testing has high sensitivity (97%) for SLE but limited specificity (34%) [1]. Thus, a positive ANA test does not exclude other autoimmune diseases with similar clinical features [2]. Testing for specific antibodies in a panel may expedite the evaluation of SLE but is generally not recommended [3].

The results of this test should be interpreted in the context of pertinent clinical and family history and physical examination findings.


Production Schedule

Sites Performed
  • Quest - Chantilly
Days Performed
Monday
Tuesday
Wednesday
Thursday
Friday
Departments
  • Sendouts - Miscellaneous
Turn Around Time

1 to 4 days


Coding & Compliance

CDM

00913333


CPT Coding

83516, 86038, 86160 (x2), 86225, 86235 (x5), 86376, 86431, If the ANA Screen, IFA is positive, then ANA Titer and Pattern will be performed at an additional charge (CPT code(s): 86039)